An improved collagen zymography approach for evaluating the collagenases MMP-1, MMP-8, and MMP-13

Collagen zymography is an SDS-PAGE-based method for detecting both the proenzyme and active forms of collagenases. Although collagen zymography is used for assessment of the matrix metalloproteinases MMP-1 and MMP-13, it can be difficult to detect these collagenases due to technical issues. Moreover...

Description complète

Détails bibliographiques
Publié dans:BioTechniques. - 1993. - 63(2017), 4 vom: 01. Okt., Seite 174-180
Auteur principal: Inanc, Seniz (Auteur)
Autres auteurs: Keles, Didem, Oktay, Gulgun
Format: Article en ligne
Langue:English
Publié: 2017
Accès à la collection:BioTechniques
Sujets:Journal Article Validation Study Collagen Type I Enzyme Precursors Isoenzymes Recombinant Proteins MMP13 protein, human EC 3.4.24.- Matrix Metalloproteinase 13 MMP8 protein, human plus... EC 3.4.24.34 Matrix Metalloproteinase 8 MMP1 protein, human EC 3.4.24.7 Matrix Metalloproteinase 1