Genetic dissection of Arabidopsis MAP kinase phosphatase 1-dependent PAMP-induced transcriptional responses

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 68(2017), 18 vom: 02. Nov., Seite 5207-5220
1. Verfasser:	Jiang, Lingyan (VerfasserIn)
Weitere Verfasser:	Wan, Ying, Anderson, Jeffrey C, Hou, Jie, Islam, Soliman M, Cheng, Jianlin, Peck, Scott C
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2017
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Arabidopsis MKP1 MPK6 RNAseq mitogen-activated protein kinase pathogen-associated molecular pattern (PAMP) phosphatase transcriptome Arabidopsis Proteins mehr... Pathogen-Associated Molecular Pattern Molecules Receptors, Pattern Recognition MPK6 protein, Arabidopsis EC 2.7.11.24 Mitogen-Activated Protein Kinases MKP1 protein, Arabidopsis EC 3.1.3.48 Protein Tyrosine Phosphatases

Beschreibung
Zusammenfassung:	© Society for Experimental Biology 2017. Plant immunity is initiated by extracellular detection of pathogen-associated molecular patterns (PAMPs) through surface-localized pattern recognition receptors (PRRs). PRR activation induces many responses including the activation of mitogen-activated protein kinases (MAPKs) that ultimately limit bacterial growth. Previous work identified Arabidopsis MAP kinase phosphatase 1 (MKP1) as a negative regulator of signaling pathways required for some, but not all, of PAMP-initiated responses. Specifically, loss of MAPK MPK6 in an mkp1 background suppressed a subset of the mkp1-dependent biological phenotypes, indicating the requirement for MPK6 in MKP1-dependent signaling. To further genetically separate the outputs of PAMP-responsive signaling pathways, we performed a transcriptome analysis in Arabidopsis wild type, mkp1 and mkp1 mpk6 seedlings treated with the bacterially derived PAMP elf26 for 0, 30, and 90 min. Using differential genetic and temporal clustering analyses between and within genotypes, we identified and separated 6963 elf26-responsive transcripts based on both genetic requirements of MKP1 (with or without a requirement for MPK6) and temporal transcriptional accumulation patterns, and some of these novel response markers were validated by qRT-PCR over a more extended time course. Taken together, our transcriptome analysis provides novel information for delineating PAMP signaling pathways
Beschreibung:	Date Completed 05.07.2018 Date Revised 13.11.2018 published: Print Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erx335