Overexpression of Notch ligand Delta-like-1 by dendritic cells enhances their immunoregulatory capacity and exerts antiallergic effects on Th2-mediated allergic asthma in mice

Copyright © 2017 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 187(2018) vom: 15. Feb., Seite 58-67
1. Verfasser: Lee, Chen-Chen (VerfasserIn)
Weitere Verfasser: Lin, Chu-Lun, Leu, Sy-Jye, Lee, Yueh-Lun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Adenovirus Allergic asthma Delta-like-1 Dendritic cells Th1 Th2 Calcium-Binding Proteins Dlk1 protein, mouse mehr... Intercellular Signaling Peptides and Proteins Interleukin-12 187348-17-0 Immunoglobulin E 37341-29-0 Ovalbumin 9006-59-1
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245 1 0 |a Overexpression of Notch ligand Delta-like-1 by dendritic cells enhances their immunoregulatory capacity and exerts antiallergic effects on Th2-mediated allergic asthma in mice 
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520 |a Dendritic cells (DCs) are professional antigen-presenting cells, and Notch ligand Delta-like-1 (DLL1) on DCs was implicated in type 1T helper (Th1) differentiation. In this study, we produced genetically engineered bone marrow-derived DCs that expressed DLL1 (DLL1-DCs) by adenoviral transduction. DLL1-DCs exerted a fully mature phenotype, and had positive effects on expression levels of interleukin (IL)-12 and costimulatory molecules. Coculture of allogeneic T cells with ovalbumin (OVA)-pulsed DLL1-DCs enhanced T cell proliferative responses and promoted Th1 cell differentiation. Furthermore, adoptive transfer of OVA-stimulated DLL1-DCs into asthmatic mice alleviated the cardinal features of allergic asthma, including immunoglobulin E (IgE) production, airway hyperresponsiveness (AHR), airway inflammation, and production of Th2-type cytokines. Notably, enhanced levels of the Th1-biased IgG2a response and interferon (IFN)-γ production were observed in these mice. Taken together, these data indicate that DLL1-DCs promoted Th1 cell development to alter the Th1/Th2 ratio and ameliorate Th2-mediated allergic asthma in mice 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Adenovirus 
650 4 |a Allergic asthma 
650 4 |a Delta-like-1 
650 4 |a Dendritic cells 
650 4 |a Th1 
650 4 |a Th2 
650 7 |a Calcium-Binding Proteins  |2 NLM 
650 7 |a Dlk1 protein, mouse  |2 NLM 
650 7 |a Intercellular Signaling Peptides and Proteins  |2 NLM 
650 7 |a Interleukin-12  |2 NLM 
650 7 |a 187348-17-0  |2 NLM 
650 7 |a Immunoglobulin E  |2 NLM 
650 7 |a 37341-29-0  |2 NLM 
650 7 |a Ovalbumin  |2 NLM 
650 7 |a 9006-59-1  |2 NLM 
700 1 |a Lin, Chu-Lun  |e verfasserin  |4 aut 
700 1 |a Leu, Sy-Jye  |e verfasserin  |4 aut 
700 1 |a Lee, Yueh-Lun  |e verfasserin  |4 aut 
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856 4 0 |u http://dx.doi.org/10.1016/j.clim.2017.10.005  |3 Volltext 
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