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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erx331
|2 doi
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|a pubmed24n1223.xml
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|a (DE-627)NLM277050782
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|a (NLM)29036360
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Brown, Daniel
|e verfasserin
|4 aut
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|a Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.07.2018
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|a Date Revised 13.12.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Natural rubber (polyisoprene) from the rubber tree Hevea brasiliensis is synthesized by specialized cells called laticifers. It is not clear how rubber particles arise, although one hypothesis is that they derive from the endoplasmic reticulum (ER) membrane. Here we cloned the genes encoding four key proteins found in association with rubber particles and studied their intracellular localization by transient expression in Nicotiana benthamiana leaves. We show that, while the cis-prenyltransferase (CPT), responsible for the synthesis of long polyisoprene chains, is a soluble, cytosolic protein, other rubber particle proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and Hevea rubber transferase 1-REF bridging protein (HRBP) are associated with the endoplasmic reticulum (ER). We also show that SRPP can recruit CPT to the ER and that interaction of CPT with HRBP leads to both proteins relocating to the plasma membrane. We discuss these results in the context of the biogenesis of rubber particles
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|a Journal Article
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|a Biogenesis
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|a Hevea
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|a endoplasmic reticulum
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|a latex
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|a particle
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|a plant
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|a rubber
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|a Antigens, Plant
|2 NLM
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|a Plant Proteins
|2 NLM
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|a SRPP protein, Hevea brasiliensis
|2 NLM
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|a Rubber
|2 NLM
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|a 9006-04-6
|2 NLM
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|a Transferases
|2 NLM
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|a EC 2.-
|2 NLM
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|a cis-prenyl transferase
|2 NLM
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|a EC 2.5.1.-
|2 NLM
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1 |
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|a Feeney, Mistianne
|e verfasserin
|4 aut
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1 |
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|a Ahmadi, Mathin
|e verfasserin
|4 aut
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1 |
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|a Lonoce, Chiara
|e verfasserin
|4 aut
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1 |
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|a Sajari, Roslinda
|e verfasserin
|4 aut
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|a Di Cola, Alessandra
|e verfasserin
|4 aut
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|a Frigerio, Lorenzo
|e verfasserin
|4 aut
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773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 18 vom: 02. Nov., Seite 5045-5055
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:68
|g year:2017
|g number:18
|g day:02
|g month:11
|g pages:5045-5055
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|u http://dx.doi.org/10.1093/jxb/erx331
|3 Volltext
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|d 68
|j 2017
|e 18
|b 02
|c 11
|h 5045-5055
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