DNA demethylation activates genes in seed maternal integument development in rice (Oryza sativa L.)

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 120(2017) vom: 01. Nov., Seite 169-178
1. Verfasser:	Wang, Yifeng (VerfasserIn)
Weitere Verfasser:	Lin, Haiyan, Tong, Xiaohong, Hou, Yuxuan, Chang, Yuxiao, Zhang, Jian
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2017
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article DNA methylation Demethylation Rice (Oryza sativa L.) Seed integument development DNA, Plant

Beschreibung
Zusammenfassung:	Copyright © 2017 Elsevier Masson SAS. All rights reserved. DNA methylation is an important epigenetic modification that regulates various plant developmental processes. Rice seed integument determines the seed size. However, the role of DNA methylation in its development remains largely unknown. Here, we report the first dynamic DNA methylomic profiling of rice maternal integument before and after pollination by using a whole-genome bisulfite deep sequencing approach. Analysis of DNA methylation patterns identified 4238 differentially methylated regions underpin 4112 differentially methylated genes, including GW2, DEP1, RGB1 and numerous other regulators participated in maternal integument development. Bisulfite sanger sequencing and qRT-PCR of six differentially methylated genes revealed extensive occurrence of DNA hypomethylation triggered by double fertilization at IAP compared with IBP, suggesting that DNA demethylation might be a key mechanism to activate numerous maternal controlling genes. These results presented here not only greatly expanded the rice methylome dataset, but also shed novel insight into the regulatory roles of DNA methylation in rice seed maternal integument development
Beschreibung:	Date Completed 29.05.2018 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2017.09.018