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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.7b02804
|2 doi
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|a eng
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| 100 |
1 |
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|a Gao, Yuan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Single-Janus Rod Tracking Reveals the "Rock-and-Roll" of Endosomes in Living Cells
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|c 2018
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|a Text
|b txt
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|a ƒaComputermedien
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|a Date Completed 11.09.2018
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|a Date Revised 10.12.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Endosomes in cells are known to move directionally along microtubules, but their rotational dynamics have rarely been investigated. Even less is known, specifically, about the rotation of nonspherical endosomes. Here we report a single-Janus rod rotational tracking study to reveal the rich rotational dynamics of rod-shaped endosomes in living cells. The rotational reporters were Janus rods that display patches of different fluorescent colors on opposite sides along their long axes. When the Janus rods are wrapped tightly inside endosomes, their shape and optical anisotropy allow the simultaneous measurements of all three rotational angles (in-plane, out-of-plane, and longitudinal) and the translational motion of single endosomes with high spatiotemporal resolutions. We demonstrate that endosomes undergo in-plane rotation and rolling during intracellular transport and that such rotational dynamics are driven by rapid microtubule fluctuations. We reveal for the first time the "rock-and-roll" of endosomes in living cells and how the intracellular environment modifies such rotational dynamics. This study demonstrates a unique application of Janus particles as imaging probes in the elucidation of fundamental biological questions
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Silicon Dioxide
|2 NLM
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| 650 |
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|a 7631-86-9
|2 NLM
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| 700 |
1 |
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|a Anthony, Stephen M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yi, Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Wenqian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Yanqi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Yan
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 34(2018), 3 vom: 23. Jan., Seite 1151-1158
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:34
|g year:2018
|g number:3
|g day:23
|g month:01
|g pages:1151-1158
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|u http://dx.doi.org/10.1021/acs.langmuir.7b02804
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