Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

Copyright © 2017. Published by Elsevier Inc.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 183(2017) vom: 05. Okt., Seite 325-335
1. Verfasser: Duffy, Darragh (VerfasserIn)
Weitere Verfasser: Rouilly, Vincent, Braudeau, Cecile, Corbière, Véronique, Djebali, Raouf, Ungeheuer, Marie-Noelle, Josien, Regis, LaBrie, Samuel T, Lantz, Olivier, Louis, Delphine, Martinez-Caceres, Eva, Mascart, Francoise, Ruiz de Morales, Jose G, Ottone, Catherine, Redjah, Lydia, Guen, Nina Salabert-Le, Savenay, Alain, Schmolz, Manfred, Toubert, Antoine, Albert, Matthew L, Multinational FOCIS Centers of Excellence
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Multicenter Study Research Support, Non-U.S. Gov't Cytokines Functional immune responses Immunomonitoring Multi-center clinical studies PBMCs Whole blood Antibodies mehr... Biomarkers CD3 Complex CD8 Antigens Lipopolysaccharides
LEADER 01000naa a22002652 4500
001 NLM276136306
003 DE-627
005 20231225011317.0
007 cr uuu---uuuuu
008 231225s2017 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.clim.2017.09.019  |2 doi 
028 5 2 |a pubmed24n0920.xml 
035 |a (DE-627)NLM276136306 
035 |a (NLM)28943400 
035 |a (PII)S1521-6616(17)30198-5 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Duffy, Darragh  |e verfasserin  |4 aut 
245 1 0 |a Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study 
264 1 |c 2017 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 13.12.2017 
500 |a Date Revised 06.02.2018 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Copyright © 2017. Published by Elsevier Inc. 
520 |a Functional immune responses are increasingly important for clinical studies, providing in depth biomarker information to assess immunotherapy or vaccination. Incorporating functional immune assays into routine clinical practice has remained limited due to challenges in standardizing sample preparation. We recently described the use of a whole blood syringe-based system, TruCulture®, which permits point-of-care standardized immune stimulation. Here, we report on a multi-center clinical study in seven FOCIS Centers of Excellence to directly compare TruCulture to conventional PBMC methods. Whole blood and PBMCs from healthy donors were exposed to LPS, anti-CD3 anti-CD28 antibodies, or media alone. 55 protein analytes were analyzed centrally by Luminex multi-analyte profiling in a CLIA-certified laboratory. TruCulture responses showed greater reproducibility and improved the statistical power for monitoring differential immune response activation. The use of TruCulture addresses a major unmet need through a robust and flexible method for immunomonitoring that can be reproducibly applied in multi-center clinical studies 
520 |a ONE SENTENCE SUMMARY: A multi-center study revealed greater reproducibility from whole blood stimulation systems as compared to PBMC stimulation for studying induced immune responses 
650 4 |a Journal Article 
650 4 |a Multicenter Study 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Cytokines 
650 4 |a Functional immune responses 
650 4 |a Immunomonitoring 
650 4 |a Multi-center clinical studies 
650 4 |a PBMCs 
650 4 |a Whole blood 
650 7 |a Antibodies  |2 NLM 
650 7 |a Biomarkers  |2 NLM 
650 7 |a CD3 Complex  |2 NLM 
650 7 |a CD8 Antigens  |2 NLM 
650 7 |a Cytokines  |2 NLM 
650 7 |a Lipopolysaccharides  |2 NLM 
700 1 |a Rouilly, Vincent  |e verfasserin  |4 aut 
700 1 |a Braudeau, Cecile  |e verfasserin  |4 aut 
700 1 |a Corbière, Véronique  |e verfasserin  |4 aut 
700 1 |a Djebali, Raouf  |e verfasserin  |4 aut 
700 1 |a Ungeheuer, Marie-Noelle  |e verfasserin  |4 aut 
700 1 |a Josien, Regis  |e verfasserin  |4 aut 
700 1 |a LaBrie, Samuel T  |e verfasserin  |4 aut 
700 1 |a Lantz, Olivier  |e verfasserin  |4 aut 
700 1 |a Louis, Delphine  |e verfasserin  |4 aut 
700 1 |a Martinez-Caceres, Eva  |e verfasserin  |4 aut 
700 1 |a Mascart, Francoise  |e verfasserin  |4 aut 
700 1 |a Ruiz de Morales, Jose G  |e verfasserin  |4 aut 
700 1 |a Ottone, Catherine  |e verfasserin  |4 aut 
700 1 |a Redjah, Lydia  |e verfasserin  |4 aut 
700 1 |a Guen, Nina Salabert-Le  |e verfasserin  |4 aut 
700 1 |a Savenay, Alain  |e verfasserin  |4 aut 
700 1 |a Schmolz, Manfred  |e verfasserin  |4 aut 
700 1 |a Toubert, Antoine  |e verfasserin  |4 aut 
700 1 |a Albert, Matthew L  |e verfasserin  |4 aut 
700 0 |a Multinational FOCIS Centers of Excellence  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Clinical immunology (Orlando, Fla.)  |d 1999  |g 183(2017) vom: 05. Okt., Seite 325-335  |w (DE-627)NLM098196855  |x 1521-7035  |7 nnns 
773 1 8 |g volume:183  |g year:2017  |g day:05  |g month:10  |g pages:325-335 
856 4 0 |u http://dx.doi.org/10.1016/j.clim.2017.09.019  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_11 
912 |a GBV_ILN_24 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 183  |j 2017  |b 05  |c 10  |h 325-335