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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2017.09.011
|2 doi
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|a pubmed24n1223.xml
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|a (DE-627)NLM276125363
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|a (NLM)28942291
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|a (PII)S0981-9428(17)30302-9
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|a DE-627
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|e rakwb
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|a eng
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|a Kumari, Kumkum
|e verfasserin
|4 aut
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|a T-homoeolog specific plasma membrane protein 3 [Nt(t)PMP3-2] in polyploid Nicotiana tabacum shows conserved alternative splicing, derived from extant Nicotiana tomentosiformis parent
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 26.12.2017
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2017 Elsevier Masson SAS. All rights reserved.
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|a Abiotic stress induced plasma membrane protein 3 (PMP3) genes occur as multigene families in plants, coding for hydrophobic proteins. Group I PMP3s code for shorter ORFs while Group II PMP3s code for proteins with C-terminal extensions. Allotetraploid Nicotiana tabacum (SSTT; 2n = 48) derives its parentage from extant ancestors related to Nicotiana sylvestris (SS) and Nicotiana tomentosiformis (TT). Polyploidization triggers complex genetic and epigenetic changes, often leading to homoeolog-specific retention or loss of function, sub-functionalization or neo-functionalization. Genomic sequences of Nt(t)PMP3-1/Nt(t)PMP3-2 cloned from N. tabacum show near identity with N. tomentosiformis NtoPMP3-1/NtoPMP3-2 genomic sequences respectively (distinct from N. sylvestris NsPMP3-1/NsPMP3-2 genomic regions). RT-PCR with exon 1,2 primer pairs amplified only single fragments for Nt(t)PMP3-1 and Nt(t)PMP3-2. In contrast, for Nt(t)PMP3-2, three variants were detected using exon 2,3 primers by RT-PCR. Cloning revealed (i) a transcript coding for a Group I PMP3 [Nt(t)PMP3-2CS], (ii) a transcript with complete retention of the second intron [Nt(t)PMP3-2IR] and (iii) a transcript with an alternative (exon 2) 5' splice site [Nt(t)PMP3-2AS], coding for a longer protein, similar to ORFs of Group II PMP3 genes. All three Nt(t)PMP3-2 variants have conserved counterparts in the N. tomentosiformis transcriptome, suggesting the transcriptional machinery governing alternative splicing of Nt(t)PMP3-2 in N. tabacum has conserved origins, derived from a N. tomenosiformis lineage. The above data shows alternative splicing of PMP3 genes contributes to transcript and ORF diversity in plants. All three Nt(t)PMP3-2 splice variants show increased root-specific expression. Implications of Nt(t)PMP3-2 alternative splicing on transcript stability and ORF features are discussed
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|a Journal Article
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|a Alternative splicing
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|a Homoeologs
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|a PMP3
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|a Polyploidy
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|a RT-PCR
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|a Tobacco
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|a Membrane Proteins
|2 NLM
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|a Jegadeeson, Vidya
|e verfasserin
|4 aut
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|a Suji, S
|e verfasserin
|4 aut
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|a Venkataraman, Gayatri
|e verfasserin
|4 aut
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|a Parida, Ajay
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 119(2017) vom: 25. Okt., Seite 338-345
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:119
|g year:2017
|g day:25
|g month:10
|g pages:338-345
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|u http://dx.doi.org/10.1016/j.plaphy.2017.09.011
|3 Volltext
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