|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM276075366 |
| 003 |
DE-627 |
| 005 |
20231225011151.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/acs.langmuir.7b02641
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0920.xml
|
| 035 |
|
|
|a (DE-627)NLM276075366
|
| 035 |
|
|
|a (NLM)28937222
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Moringo, Nicholas A
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Variable Lysozyme Transport Dynamics on Oxidatively Functionalized Polystyrene Films
|
| 264 |
|
1 |
|c 2017
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 28.01.2019
|
| 500 |
|
|
|a Date Revised 28.01.2019
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Tuning protein adsorption dynamics at polymeric interfaces is of great interest to many biomedical and material applications. Functionalization of polymer surfaces is a common method to introduce application-specific surface chemistries to a polymer interface. In this work, single-molecule fluorescence microscopy is utilized to determine the adsorption dynamics of lysozyme, a well-studied antibacterial protein, at the interface of polystyrene oxidized via UV exposure and oxygen plasma and functionalized by ligand grafting to produce varying degrees of surface hydrophilicity, surface roughness, and induced oxygen content. Single-molecule tracking indicates lysozyme loading capacities, and surface mobility at the polymer interface is hindered as a result of all functionalization techniques. Adsorption dynamics of lysozyme depend on the extent and the specificity of the oxygen functionalities introduced to the polystyrene surface. Hindered adsorption and mobility are dominated by hydrophobic effects attributed to water hydration layer formation at the functionalized polystyrene surfaces
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a Polystyrenes
|2 NLM
|
| 650 |
|
7 |
|a Muramidase
|2 NLM
|
| 650 |
|
7 |
|a EC 3.2.1.17
|2 NLM
|
| 700 |
1 |
|
|a Shen, Hao
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Tauzin, Lawrence J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Wenxiao
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bishop, Logan D C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Landes, Christy F
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 33(2017), 41 vom: 17. Okt., Seite 10818-10828
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:33
|g year:2017
|g number:41
|g day:17
|g month:10
|g pages:10818-10828
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/acs.langmuir.7b02641
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 33
|j 2017
|e 41
|b 17
|c 10
|h 10818-10828
|