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231225s2018 xx |||||o 00| ||eng c |
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|a 10.5713/ajas.17.0552
|2 doi
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|a pubmed24n0919.xml
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|a (DE-627)NLM275910792
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|a (NLM)28920412
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Park, Tansol
|e verfasserin
|4 aut
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| 245 |
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|a Molecular cloning, purification, expression, and characterization of β-1, 4-endoglucanase gene (Cel5A) from Eubacterium cellulosolvens sp. isolated from Holstein steers' rumen
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 01.10.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a OBJECTIVE: This study was conducted to isolate the cellulolytic microorganism from the rumen of Holstein steers and characterize endoglucanase gene (Cel5A) from the isolated microorganism
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|a METHODS: To isolate anaerobic microbes having endoglucanase, rumen fluid was obtained from Holstein steers fed roughage diet. The isolated anaerobic bacteria had 98% similarity with Eubacterium cellulosolvens (E. cellulosolvens) Ce2 (Accession number: AB163733). The Cel5A from isolated E. cellulolsovens sp. was cloned using the published genome sequence and expressed through the Escherichia coli BL21
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|a RESULTS: The maximum activity of recombinant Cel5A (rCel5A) was observed at 50°C and pH 4.0. The enzyme was constant at the temperature range of 20°C to 40°C but also, at the pH range of 3 to 9. The metal ions including Ca2+, K+, Ni2+, Mg2+, and Fe2+ increased the endoglucanase activity but the addition of Mn2+, Cu2+, and Zn2+ decreased. The Km and Vmax value of rCel5A were 14.05 mg/mL and 45.66 μmol/min/mg. Turnover number, Kcat and catalytic efficiency, Kcat/Km values of rCel5A was 96.69 (s-1) and 6.88 (mL/mg/s), respectively
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|a CONCLUSION: Our results indicated that rCel5A of E. cellulosolvens isolated from Holstein steers had a broad pH range with high stability under various conditions, which might be one of the beneficial characteristics of this enzyme for possible industrial application
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|a Journal Article
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|a Endoglucanase
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|a Eubacterium cellulosolvens sp.
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|a Optimal Condition
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| 650 |
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|a Rumen Bacteria
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|a Seo, Seongwon
|e verfasserin
|4 aut
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|a Shin, Teaksoon
|e verfasserin
|4 aut
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|a Cho, Byung-Wook
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cho, Seongkeun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, Byeongwoo
|e verfasserin
|4 aut
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| 700 |
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|a Lee, Seyoung
|e verfasserin
|4 aut
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| 700 |
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|a Ha, Jong K
|e verfasserin
|4 aut
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| 700 |
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|a Seo, Jakyeom
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Asian-Australasian journal of animal sciences
|d 1998
|g 31(2018), 4 vom: 25. Apr., Seite 607-615
|w (DE-627)NLM098195883
|x 1011-2367
|7 nnns
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| 773 |
1 |
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|g volume:31
|g year:2018
|g number:4
|g day:25
|g month:04
|g pages:607-615
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|u http://dx.doi.org/10.5713/ajas.17.0552
|3 Volltext
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