Comparison of bacterial communities in leachate from decomposing bovine carcasses

OBJECTIVE: Burial is associated with environmental effects such as the contamination of ground or surface water with biological materials generated during the decomposition process. Therefore, bacterial communities in leachates originating from the decomposing bovine carcasses were investigated

Bibliographische Detailangaben
Veröffentlicht in:Asian-Australasian journal of animal sciences. - 1998. - 30(2017), 11 vom: 25. Nov., Seite 1660-1666
1. Verfasser: Yang, Seung Hak (VerfasserIn)
Weitere Verfasser: Ahn, Hee Kwon, Kim, Bong Soo, Chang, Sun Sik, Chung, Ki Yong, Lee, Eun Mi, Ki, Kwang Seok, Kwon, Eung Gi
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Asian-Australasian journal of animal sciences
Schlagworte:Journal Article Bacterial Community Decomposition Firmicutes Hanwoo Leachate Pyrosequencing
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520 |a OBJECTIVE: Burial is associated with environmental effects such as the contamination of ground or surface water with biological materials generated during the decomposition process. Therefore, bacterial communities in leachates originating from the decomposing bovine carcasses were investigated 
520 |a METHODS: To understand the process of bovine (Hanwoo) carcass decomposition, we simulated burial using a lab-scale reactor with a volume of 5.15 m3. Leachate samples from 3 carcasses were collected using a peristaltic pump once a month for a period of 5 months, and bacterial communities in samples were identified by pyrosequencing of the 16S rRNA gene 
520 |a RESULTS: We obtained a total of 110,442 reads from the triplicate samples of various sampling time points (total of 15 samples), and found that the phylum Firmicutes was dominant at most sampling times. Differences in the bacterial communities at the various time points were observed among the triplicate samples. The bacterial communities sampled at 4 months showed the most different compositions. The genera Pseudomonas and Psychrobacter in the phylum Proteobacteria were dominant in all of the samples obtained after 3 months. Bacillaceae, Clostridium, and Clostridiales were found to be predominant after 4 months in the leachate from one carcass, whereas Planococcaceae was found to be a dominant in samples obtained at the first and second months from the other two carcasses. The results showed that potentially pathogenic microbes such as Clostridium derived from bovine leachate could dominate the soil environment of a burial site 
520 |a CONCLUSION: Our results indicated that the composition of bacterial communities in leachates of a decomposing bovine shifted continuously during the experimental period, with significant changes detected after 4 months of burial 
650 4 |a Journal Article 
650 4 |a Bacterial Community 
650 4 |a Decomposition 
650 4 |a Firmicutes 
650 4 |a Hanwoo 
650 4 |a Leachate 
650 4 |a Pyrosequencing 
700 1 |a Ahn, Hee Kwon  |e verfasserin  |4 aut 
700 1 |a Kim, Bong Soo  |e verfasserin  |4 aut 
700 1 |a Chang, Sun Sik  |e verfasserin  |4 aut 
700 1 |a Chung, Ki Yong  |e verfasserin  |4 aut 
700 1 |a Lee, Eun Mi  |e verfasserin  |4 aut 
700 1 |a Ki, Kwang Seok  |e verfasserin  |4 aut 
700 1 |a Kwon, Eung Gi  |e verfasserin  |4 aut 
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773 1 8 |g volume:30  |g year:2017  |g number:11  |g day:25  |g month:11  |g pages:1660-1666 
856 4 0 |u http://dx.doi.org/10.5713/ajas.17.0553  |3 Volltext 
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