Direct quantification of protein glycan phosphorylation

Phosphorylation is an important post-translational modification (PTM) of proteins and a critical quality attribute for protein therapeutics, especially if it is required for protein function or sub-cellular targeting. Most current methods to quantify phosphorylation are time-consuming, indirect, or...

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Veröffentlicht in:BioTechniques. - 1993. - 63(2017), 3 vom: 01. Sept., Seite 117-123
1. Verfasser: Ketcham, Stephanie A (VerfasserIn)
Weitere Verfasser: Ashraf, Muhammad, Madhavarao, Chikkathur N
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. glycosylation lysosomal enzymes mannose-6-phosphate phospho-glycans phosphorylation 1,3-bis(bis(pyridin-2-ylmethyl)amino)propan-2-ol Amino Acids mehr... Polysaccharides Pyridines Glucuronidase EC 3.2.1.31 Cathepsin D EC 3.4.23.5