Spectral phasor analysis reveals altered membrane order and function of root hair cells in Arabidopsis dry2/sqe1-5 drought hypersensitive mutant

Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 119(2017) vom: 30. Okt., Seite 224-231
1. Verfasser: Sena, Florencia (VerfasserIn)
Weitere Verfasser: Sotelo-Silveira, Mariana, Astrada, Soledad, Botella, Miguel A, Malacrida, Leonel, Borsani, Omar
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Arabidopsis thaliana Drought hypersensibility Membranes fluidity SQE1 Spectral phasor Arabidopsis Proteins Sitosterols gamma-sitosterol 5LI01C78DD mehr... Stigmasterol 99WUK5D0Y8 Squalene Monooxygenase EC 1.14.14.17
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245 1 0 |a Spectral phasor analysis reveals altered membrane order and function of root hair cells in Arabidopsis dry2/sqe1-5 drought hypersensitive mutant 
264 1 |c 2017 
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500 |a Date Completed 26.12.2017 
500 |a Date Revised 30.09.2020 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Copyright © 2017 Elsevier Masson SAS. All rights reserved. 
520 |a Biological membranes allow the regulation of numerous cellular processes, which are affected when unfavorable environmental factors are perceived. Lipids and proteins are the principal components of biological membranes. Each lipid has unique biophysical properties, and, therefore the lipid composition of the membrane is critical to maintaining the bilayer structure and functionality. Membrane composition and integrity are becoming the focus of studies aiming to understand how plants adapt to its environment. In this study, using a combination of di-4-ANEPPDHQ fluorescence and spectral phasor analysis, we report that the drought hypersensitive/squalene epoxidase (dry2/sqe1-5) mutant with reduced major sterols such as sitosterol and stigmasterol in roots presented higher membrane fluidity than the wild type. Moreover, analysis of endomembrane dynamics showed that vesicle formation was affected in dry2/sqe1-5. Further analysis of proteins associated with sterol rich micro domains showed that dry2/sqe1-5 presented micro domains function altered 
650 4 |a Journal Article 
650 4 |a Arabidopsis thaliana 
650 4 |a Drought hypersensibility 
650 4 |a Membranes fluidity 
650 4 |a SQE1 
650 4 |a Spectral phasor 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Sitosterols  |2 NLM 
650 7 |a gamma-sitosterol  |2 NLM 
650 7 |a 5LI01C78DD  |2 NLM 
650 7 |a Stigmasterol  |2 NLM 
650 7 |a 99WUK5D0Y8  |2 NLM 
650 7 |a Squalene Monooxygenase  |2 NLM 
650 7 |a EC 1.14.14.17  |2 NLM 
700 1 |a Sotelo-Silveira, Mariana  |e verfasserin  |4 aut 
700 1 |a Astrada, Soledad  |e verfasserin  |4 aut 
700 1 |a Botella, Miguel A  |e verfasserin  |4 aut 
700 1 |a Malacrida, Leonel  |e verfasserin  |4 aut 
700 1 |a Borsani, Omar  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 119(2017) vom: 30. Okt., Seite 224-231  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:119  |g year:2017  |g day:30  |g month:10  |g pages:224-231 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2017.08.017  |3 Volltext 
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