Infection with a novel derogenid trematode in a Flap-necked chameleon (Chamaeleo dilepis)

© 2017 American Society for Veterinary Clinical Pathology.

Bibliographische Detailangaben
Veröffentlicht in:Veterinary clinical pathology. - 1975. - 46(2017), 4 vom: 01. Dez., Seite 629-634
1. Verfasser: Collicutt, Nancy B (VerfasserIn)
Weitere Verfasser: Stacy, Nicole I, Walden, Heather Stockdale, Childress, April, Dill, Jennifer, Anderson, Marita, Wellehan, James F X Jr
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Veterinary clinical pathology
Schlagworte:Case Reports Halipegus Cytology digenean fluke
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520 |a A 2-year-old female Flap-necked chameleon (Chamaeleo dilepis) was presented with a history of anorexia. On physical examination, three 11×4×2 mm, light tan-pink, raised, gelatinous, freely movable, poorly demarcated, raised nodules were identified within the oral cavity. An impression smear contained multiple, light blue to green structures exhibiting an 18×35 μm, elliptical-shaped anterior portion and a single 1×150 μm filament with a tapered end. Initial differential etiologies included trematode ova, flagellate protozoa, fungal sporangia, plant material, and sperm. Fungal culture yielded no growth. The structures described above did not stain with Gomori methenamine silver stain. A calcofluor white stain yielded positive fluorescence. The DNA was extracted from the impression smears and pan-trematode PCR was performed; products were directly sequenced and phylogenetic analysis revealed maximum similarity with a digenean trematode in the family Derogenidae that was distinct from other trematodes for which a reference sequence was available. To our knowledge, this is the first description of cytologic features of trematode ova from an oral lesion in a chameleon 
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700 1 |a Walden, Heather Stockdale  |e verfasserin  |4 aut 
700 1 |a Childress, April  |e verfasserin  |4 aut 
700 1 |a Dill, Jennifer  |e verfasserin  |4 aut 
700 1 |a Anderson, Marita  |e verfasserin  |4 aut 
700 1 |a Wellehan, James F X  |c Jr  |e verfasserin  |4 aut 
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