High-density ddRAD linkage and yield-related QTL mapping delimits a chromosomal region responsible for oil content in rapeseed (Brassica napus L.)

Rapeseed (Brassica napus L.) is one of the most important oil crops almost all over the world. Seed-related traits, including oil content (OC), silique length (SL), seeds per silique (SS), and seed weight (SW), are primary targets for oil yield improvement. To dissect the genetic basis of these trai...

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Veröffentlicht in:Breeding science. - 1998. - 67(2017), 3 vom: 25. Juni, Seite 296-306
1. Verfasser: Chen, Jun (VerfasserIn)
Weitere Verfasser: Wang, Bo, Zhang, Yueli, Yue, Xiaopeng, Li, Zhaohong, Liu, Kede
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article Brassica napus oil content quantitative trait locus restriction site associated DNA yield-related trait
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520 |a Rapeseed (Brassica napus L.) is one of the most important oil crops almost all over the world. Seed-related traits, including oil content (OC), silique length (SL), seeds per silique (SS), and seed weight (SW), are primary targets for oil yield improvement. To dissect the genetic basis of these traits, 192 recombinant inbred lines (RILs) were derived from two parents with distinct oil content and silique length. High-density linkage map with a total length of 1610.4 cM were constructed using 1,329 double-digestion restriction site associated DNA (ddRAD) markers, 107 insertion/deletions (INDELs), and 90 well-distributed simple sequence repeats (SSRs) markers. A total of 37 consensus quantitative trait loci (QTLs) were detected for the four traits, with individual QTL explained 3.1-12.8% of the phenotypic variations. Interestingly, one OC consensus QTL (cqOCA10b) on chromosome A10 was consistently detected in all three environments, and explained 9.8% to 12.8% of the OC variation. The locus was further delimited into an approximately 614 kb genomic region, in which the flanking markers could be further evaluated for marker-assisted selection in rapeseed OC improvement and the candidate genes targeted for map-based cloning and genetic manipulation 
650 4 |a Journal Article 
650 4 |a Brassica napus 
650 4 |a oil content 
650 4 |a quantitative trait locus 
650 4 |a restriction site associated DNA 
650 4 |a yield-related trait 
700 1 |a Wang, Bo  |e verfasserin  |4 aut 
700 1 |a Zhang, Yueli  |e verfasserin  |4 aut 
700 1 |a Yue, Xiaopeng  |e verfasserin  |4 aut 
700 1 |a Li, Zhaohong  |e verfasserin  |4 aut 
700 1 |a Liu, Kede  |e verfasserin  |4 aut 
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773 1 8 |g volume:67  |g year:2017  |g number:3  |g day:25  |g month:06  |g pages:296-306 
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