Exogenous application of poly-γ-glutamic acid enhances stress defense in Brassica napus L. seedlings by inducing cross-talks between Ca2+, H2O2, brassinolide, and jasmonic acid in leaves
Copyright © 2017. Published by Elsevier Masson SAS.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 118(2017) vom: 15. Sept., Seite 460-470 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2017
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Brassica napus L. Brassinolide Ca(2+) signal H(2)O(2) Jasmonic acid Poly-γ-glutamic acid Brassinosteroids Cyclopentanes Oxylipins mehr... |
| Zusammenfassung: | Copyright © 2017. Published by Elsevier Masson SAS. Poly-γ-glutamic acid (γ-PGA) is a microbe-secreted isopeptide shown to promote growth and enhance crop stress tolerance. However, its downstream signaling pathways are unknown. Here, we studied γ-PGA-induced tolerance to salt and cold stresses. Pretreatment with γ-PGA contributed to enhance stress tolerance of canola seedlings by promoting proline accumulation and total antioxidant capacity (T-AOC) improvement. Further, Ca2+, H2O2, brassinolide, and jasmonic acid were found to be involved in the γ-PGA-induced process. First, using signal blockers, we concluded that γ-PGA activated Ca2+ fluctuations in canola seedling leaves. Second, the activated Ca2+ further elicited H2O2 production by Ca2+-binding proteins CBL9, CPK4, and CPK5. Third, the H2O2 signal promoted brassinolide and jasmonic acid biosynthesis by upregulating key genes (DWF4 and LOX2, respectively) for synthesizing these compounds. Lastly, brassinolide and jasmonic acid increased H2O2 which promoted proline accumulation and T-AOC improvement and further enhanced Ca2+-binding proteins including CaM, CBL10, and CPK9 |
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| Beschreibung: | Date Completed 26.12.2017 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2017.07.015 |