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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1002/adma.201701629
|2 doi
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|a pubmed25n0912.xml
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|a (NLM)28714191
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|e rakwb
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|a eng
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1 |
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|a Sarem, Melika
|e verfasserin
|4 aut
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| 245 |
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|a Disordered Conformation with Low Pii Helix in Phosphoproteins Orchestrates Biomimetic Apatite Formation
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.01.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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|a The interplay between noncollagenous proteins and biomineralization is widely accepted, yet the contribution of their secondary structure in mineral formation remains to be clarified. This study demonstrates a role for phosvitin, an intrinsically disordered phosphoprotein, in chick embryo skeletal development, and using circular dichroism and matrix least-squares Henderson-Hasselbalch global fitting, unravels three distinct pH-dependent secondary structures in phosvitin. By sequestering phosvitin on a biomimetic 3D insoluble cationic framework at defined pHs, access is gained to phosvitin in various conformational states. Induction of biomimetic mineralization at near physiological conditions reveals that a disordered secondary structure with a low content of PII helix is remarkably efficient at promoting calcium adsorption, and results in the formation of biomimetic hydroxyapatite through an amorphous calcium phosphate precursor. By extending this finding to phosphorylated full-length human recombinant dentin matrix protein-1 (17-513 AA), this bioinspired approach provides compelling evidence for the role of a disordered secondary structure in phosphoproteins in bone-like apatite formation
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|a Journal Article
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|a amorphous calcium phosphate
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|a biomineralization
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|a intrinsically disordered proteins
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|a non-collagenous proteins
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|a phosphorylated dentin matrix protein-1
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|a Apatites
|2 NLM
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|a Phosphoproteins
|2 NLM
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|a Durapatite
|2 NLM
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|a 91D9GV0Z28
|2 NLM
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1 |
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|a Lüdeke, Steffen
|e verfasserin
|4 aut
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1 |
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|a Thomann, Ralf
|e verfasserin
|4 aut
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1 |
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|a Salavei, Pavel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zou, Zhaoyong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Habraken, Wouter
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Masic, Admir
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shastri, V Prasad
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 29(2017), 35 vom: 27. Sept.
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|g volume:29
|g year:2017
|g number:35
|g day:27
|g month:09
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|u http://dx.doi.org/10.1002/adma.201701629
|3 Volltext
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