|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM273875353 |
| 003 |
DE-627 |
| 005 |
20250221234615.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2017.06.030
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0912.xml
|
| 035 |
|
|
|a (DE-627)NLM273875353
|
| 035 |
|
|
|a (NLM)28711790
|
| 035 |
|
|
|a (PII)S0981-9428(17)30221-8
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Wu, Yingling
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Six phenylalanine ammonia-lyases from Camellia sinensis
|b Evolution, expression, and kinetics
|
| 264 |
|
1 |
|c 2017
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 26.12.2017
|
| 500 |
|
|
|a Date Revised 30.09.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2017. Published by Elsevier Masson SAS.
|
| 520 |
|
|
|a Phenylalanine ammonia-lyase (PAL), the branch point enzyme controlling the flow of primary metabolism into second metabolism, converts the L-phenylalanine (L-Phe) to yield cinnamic acid. Based on the sequencing data available from eight transcriptome projects, six PAL genes have been screened out, cloned, and designated as CsPALa-CsPALf. The phylogenetic tree showed that CsPALs were divided into three subgroups, PALa and PALb, PALc and PALd, and PALe and PALf. All six CsPALs exhibited indiscriminate cytosolic locations in epidermis cells and mesophyll cells. Then, the expression profiles of six PAL genes were qualitatively investigated and they displayed tissue-/induced-expression specificity in several tissues or under different exogenous treatments. Furthermore, in vitro enzymatic assays showed that all six recombinant proteins were characterized by the strict substrate specificity toward L-Phe, but no activity toward L-Tyr, and they displayed subtle differences in kinetics and enzymatic properties. These results indicate that CsPALs play both distinct and overlapping roles in plant growth and responses to environmental cues
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Camellia sinensis
|
| 650 |
|
4 |
|a Evolution
|
| 650 |
|
4 |
|a Expression
|
| 650 |
|
4 |
|a Kinetics
|
| 650 |
|
4 |
|a Phenylalanine ammonia-lyase
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Phenylalanine Ammonia-Lyase
|2 NLM
|
| 650 |
|
7 |
|a EC 4.3.1.24
|2 NLM
|
| 700 |
1 |
|
|a Wang, Wenzhao
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Yanzhi
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Dai, Xinlong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ma, Guoliang
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Xing, Dawei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhu, Mengqing
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Gao, Liping
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Xia, Tao
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 118(2017) vom: 01. Sept., Seite 413-421
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
|
| 773 |
1 |
8 |
|g volume:118
|g year:2017
|g day:01
|g month:09
|g pages:413-421
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2017.06.030
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 118
|j 2017
|b 01
|c 09
|h 413-421
|