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231225s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1002/adma.201701255
|2 doi
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|a pubmed24n0911.xml
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|a (DE-627)NLM273523848
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|a (NLM)28675637
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Yu, Xiaohua
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Nanostructured Mineral Coatings Stabilize Proteins for Therapeutic Delivery
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| 264 |
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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| 500 |
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|a Date Completed 22.01.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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|a Proteins tend to lose their biological activity due to their fragile structural conformation during formulation, storage, and delivery. Thus, the inability to stabilize proteins in controlled-release systems represents a major obstacle in drug delivery. Here, a bone mineral inspired protein stabilization strategy is presented, which uses nanostructured mineral coatings on medical devices. Proteins bound within the nanostructured coatings demonstrate enhanced stability against extreme external stressors, including organic solvents, proteases, and ethylene oxide gas sterilization. The protein stabilization effect is attributed to the maintenance of protein conformational structure, which is closely related to the nanoscale feature sizes of the mineral coatings. Basic fibroblast growth factor (bFGF) released from a nanostructured mineral coating maintains its biological activity for weeks during release, while it maintains activity for less than 7 d during release from commonly used polymeric microspheres. Delivery of the growth factors bFGF and vascular endothelial growth factor using a mineral coated surgical suture significantly improves functional Achilles tendon healing in a rabbit model, resulting in increased vascularization, more mature collagen fiber organization, and a two fold improvement in mechanical properties. The findings of this study demonstrate that biomimetic interactions between proteins and nanostructured minerals provide a new, broadly applicable mechanism to stabilize proteins in the context of drug delivery and regenerative medicine
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|a Journal Article
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|a mineral coating
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4 |
|a nanostructures
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4 |
|a protein delivery
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| 650 |
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4 |
|a protein stability
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| 650 |
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4 |
|a therapeutics
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| 650 |
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|a Minerals
|2 NLM
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| 650 |
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|a Vascular Endothelial Growth Factor A
|2 NLM
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| 700 |
1 |
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|a Biedrzycki, Adam H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Khalil, Andrew S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hess, Dalton
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Umhoefer, Jennifer M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Markel, Mark D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Murphy, William L
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 29(2017), 33 vom: 12. Sept.
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
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|g volume:29
|g year:2017
|g number:33
|g day:12
|g month:09
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|u http://dx.doi.org/10.1002/adma.201701255
|3 Volltext
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