The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 216(2017), 1 vom: 07. Okt., Seite 150-162
1. Verfasser:	Müller, Heike M (VerfasserIn)
Weitere Verfasser:	Schäfer, Nadine, Bauer, Hubert, Geiger, Dietmar, Lautner, Silke, Fromm, Jörg, Riederer, Markus, Bueno, Amauri, Nussbaumer, Thomas, Mayer, Klaus, Alquraishi, Saleh A, Alfarhan, Ahmed H, Neher, Erwin, Al-Rasheid, Khaled A S, Ache, Peter, Hedrich, Rainer
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2017
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article S-type anion channel SLAC1 abscisic acid (ABA) signalling cuticle cuticular waxes date palm Phoenix dactylifera guard cells stomata Anions mehr... Chlorides Nitrates Plant Proteins RNA, Plant Waxes Abscisic Acid 72S9A8J5GW

Beschreibung
Zusammenfassung:	© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust. Date palm Phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. How are palms under such harsh environmental conditions able to limit transpirational water loss? Here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from P. dactylifera. To understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast abscisic acid (ABA) signalling and reconstituted this ABA signalosome in Xenopus oocytes. The PhoenixSLAC1-type anion channel is regulated by ABA kinase PdOST1. Energy-dispersive X-ray analysis (EDXA) demonstrated that date palm guard cells release chloride during stomatal closure. However, in Cl- medium, PdOST1 did not activate the desert plant anion channel PdSLAC1 per se. Only when nitrate was present at the extracellular face of the anion channel did the OST1-gated PdSLAC1 open, thus enabling chloride release. In the presence of nitrate, ABA enhanced and accelerated stomatal closure. Our findings indicate that, in date palm, the guard cell osmotic motor driving stomatal closure uses nitrate as the signal to open the major anion channel SLAC1. This initiates guard cell depolarization and the release of anions together with potassium
Beschreibung:	Date Completed 15.05.2018 Date Revised 08.04.2022 published: Print-Electronic CommentIn: New Phytol. 2017 Oct;216(1):5-7. - PMID 28850184 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.14672