Three-dimensional reconstruction for coherent diffraction patterns obtained by XFEL

The three-dimensional (3D) structural analysis of single particles using an X-ray free-electron laser (XFEL) is a new structural biology technique that enables observations of molecules that are difficult to crystallize, such as flexible biomolecular complexes and living tissue in the state close to...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 24(2017), Pt 4 vom: 01. Juli, Seite 727-737
1. Verfasser: Nakano, Miki (VerfasserIn)
Weitere Verfasser: Miyashita, Osamu, Jonic, Slavica, Song, Changyong, Nam, Daewoong, Joti, Yasumasa, Tama, Florence
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article Research Support, Non-U.S. Gov't X-ray free-electron laser coherent X-ray diffraction imaging single-particle analysis three-dimensional reconstruction
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520 |a The three-dimensional (3D) structural analysis of single particles using an X-ray free-electron laser (XFEL) is a new structural biology technique that enables observations of molecules that are difficult to crystallize, such as flexible biomolecular complexes and living tissue in the state close to physiological conditions. In order to restore the 3D structure from the diffraction patterns obtained by the XFEL, computational algorithms are necessary as the orientation of the incident beam with respect to the sample needs to be estimated. A program package for XFEL single-particle analysis based on the Xmipp software package, that is commonly used for image processing in 3D cryo-electron microscopy, has been developed. The reconstruction program has been tested using diffraction patterns of an aerosol nanoparticle obtained by tomographic coherent X-ray diffraction microscopy 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a X-ray free-electron laser 
650 4 |a coherent X-ray diffraction imaging 
650 4 |a single-particle analysis 
650 4 |a three-dimensional reconstruction 
700 1 |a Miyashita, Osamu  |e verfasserin  |4 aut 
700 1 |a Jonic, Slavica  |e verfasserin  |4 aut 
700 1 |a Song, Changyong  |e verfasserin  |4 aut 
700 1 |a Nam, Daewoong  |e verfasserin  |4 aut 
700 1 |a Joti, Yasumasa  |e verfasserin  |4 aut 
700 1 |a Tama, Florence  |e verfasserin  |4 aut 
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