Location and characterization of lignin in tracheid cell walls of radiata pine (Pinus radiata D. Don) compression woods

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 118(2017) vom: 05. Sept., Seite 187-198
1. Verfasser:	Zhang, Miao (VerfasserIn)
Weitere Verfasser:	Lapierre, Catherine, Nouxman, Noor Liyana, Nieuwoudt, Michel K, Smith, Bronwen G, Chavan, Ramesh R, McArdle, Brian H, Harris, Philip J
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2017
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Acridine orange Compression wood FTIR micro-spectroscopy Lignin Principal component analysis (PCA) Raman micro-spectroscopy Thioacidolysis 9005-53-2

Beschreibung
Zusammenfassung:	Copyright © 2017 Elsevier Masson SAS. All rights reserved. Tilted stems of softwoods form compression wood (CW) and opposite wood (OW) on their lower and upper sides, respectively. More is known about the most severe form of CW, severe CW (SCW), but mild CWs (MCWs) also occur widely. Two grades of MCWs, MCW1 and MCW2, as well as SCW and OW were identified in the stems of radiata pine (Pinus radiata) that had been slightly tilted. The four wood types were identified by the distribution of lignin in the tracheid walls determined by fluorescence microscopy. A solution of the fluorescent dye acridine orange (AO) (0.02% at pH 6 or 7) was shown to metachromatically stain the tracheid walls and can also be used to determine lignin distribution. The lignified walls fluoresced orange to yellow depending on the lignin concentration. Microscopically well-characterized discs (0.5 mm diameter) of the wood types were used to determine lignin concentrations and lignin monomer compositions using the acetyl bromide method and thioacidolysis, respectively. Lignin concentration and the proportion of p-hydroxyphenyl units (H-units) relative to guaiacyl (G-units) increased with CW severity, with <1% H-units in OW and up to 14% in SCW. Lignin H-units can be used as a marker for CW and CW severity. Similar discs were also examined by Raman and FTIR micro-spectroscopies coupled with principal component analysis (PCA) to determine if these techniques can be used to differentiate the four different wood types. Both techniques were able to do this, particularly Raman micro-spectroscopy
Beschreibung:	Date Completed 21.12.2017 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2017.06.012