Specificity of, and influence of hemolysis, lipemia, and icterus on serum lipase activity as measured by the v-LIP-P slide
© 2017 American Society for Veterinary Clinical Pathology.
| Veröffentlicht in: | Veterinary clinical pathology. - 1975. - 46(2017), 3 vom: 18. Sept., Seite 508-515 |
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| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2017
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| Zugriff auf das übergeordnete Werk: | Veterinary clinical pathology |
| Schlagworte: | Journal Article Hypertriglyceridemia Intralipid Spec cPL influence factors lipase Lipase EC 3.1.1.3 |
| Zusammenfassung: | © 2017 American Society for Veterinary Clinical Pathology. BACKGROUND: Measurement of pancreatic lipase in serum is being used for the diagnosis of pancreatitis OBJECTIVES: The aims of this study were to measure serum lipase activity by the v-LIP-P slide and serum canine pancreatic lipase immunoreactivity (cPLI) concentration by Spec cPL in dogs with exocrine pancreatic insufficiency (EPI) and in serum samples that were hemolyzed, lipemic, or icteric METHODS: Serum samples from 50 dogs with EPI, 8 samples spiked with canine hemoglobin, Intralipid, or ditaurobilirubin, and 8 samples from dogs with severe hypertriglyceridemia were evaluated using v-LIP-P and Spec cPL assays RESULTS: Serum lipase activity (v-LIP-P) was in the lower 20% of the RI in 58% of EPI dogs, while serum cPLI (as measured by Spec cPL) was in the lower 20% of the RI in 98% of EPI dogs. The mean (±SD) observed-to-expected ratios for serum samples spiked with canine hemoglobin, Intralipid, or ditaurobilirubin were 63.0 ± 25.1%, 489.2 ± 469.7%, and 80.2 ± 11.6% for the v-LIP-P slide, respectively, and 99.3 ± 6.8%, 96.9 ± 9.4%, and 98.7 ± 11.0% for Spec cPL, respectively. However, naturally occurring hypertriglyceridemia did not appear to have a significant effect on serum lipase activity as measured by the v-LIP-P slide CONCLUSIONS: These results show that Spec cPL is specific for pancreatic lipase, while the v-LIP-P slide is not. In addition, hemolysis and lipemia had no effect on Spec cPL, while severe icterus resulted in a slight decrease in Spec cPL. Hemolysis and icterus had a significant effect on the v-LIP-P slide. The effect of lipemia on the v-LIP-P slide cannot be conclusively established based on this study |
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| Beschreibung: | Date Completed 13.03.2018 Date Revised 13.03.2018 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1939-165X |
| DOI: | 10.1111/vcp.12517 |