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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.7b01062
|2 doi
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|a pubmed24n0910.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Shoaib, Tooba
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Assembly, Morphology, Diffusivity, and Indentation of Hydrogel-Supported Lipid Bilayers
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|c 2017
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 23.01.2019
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|a Date Revised 23.01.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Recognizing the limitations of solid-supported lipid bilayers to reproduce the behavior of cell membranes, including bendability, transmembrane protein inclusion, and virus entry, this study describes a novel biomimetic system for cell membranes with the potential to overcome these and other limitations. The developed strategy utilizes a hydrogel with tunable mechanical behavior that resembles those of living cells as the soft support for the phospholipid bilayer, while a polyelectrolyte multilayer film serves as an intermediate layer to facilitate the self-assembly of the lipid bilayer on the soft cushion. Quartz crystal microbalance studies show that, upon coming into contact with the polyelectrolyte film, vesicles fuse and rupture to yield a robust lipid bilayer. Fluorescence recovery after photobleaching confirms the formation of a membrane, while atomic force microscopy shows a low adhesion between the indenting probe and the bilayer. More importantly, in comparison to the solid-supported lipid bilayer, the response of this biomimetic system to nanoindentation demonstrates its increased mechanical stability and bendability when assembled on a soft cushion. Hence, the developed hydrogel-supported lipid bilayers can mimic biomechanical properties of cell membranes, which will enable scientists to study and to understand biophysicochemical interactions between cell membranes and extracellular entities
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Hydrogels
|2 NLM
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|a Lipid Bilayers
|2 NLM
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| 650 |
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|a Phospholipids
|2 NLM
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|a Nalam, Prathima C
|e verfasserin
|4 aut
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|a He, Yichen
|e verfasserin
|4 aut
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| 700 |
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|a Chen, Yuting
|e verfasserin
|4 aut
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|a Espinosa-Marzal, Rosa M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 33(2017), 28 vom: 18. Juli, Seite 7105-7117
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:33
|g year:2017
|g number:28
|g day:18
|g month:07
|g pages:7105-7117
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|u http://dx.doi.org/10.1021/acs.langmuir.7b01062
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