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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erx187
|2 doi
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|a pubmed25n0910.xml
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|a (DE-627)NLM273115413
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|a (NLM)28633340
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Shao-Jia
|e verfasserin
|4 aut
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| 245 |
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|a Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.02.2018
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|a Date Revised 13.11.2018
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Citric acid is the predominant organic acid of citrus fruit. Degradation of citric acid occurs during fruit development, influencing fruit acidity. Associations of CitAco3 transcripts and citric acid degradation have been reported for citrus fruit. Here, transient overexpression of CitAco3 significantly reduced the citric acid content of citrus leaves and fruits. Using dual luciferase assays, it was shown that CitNAC62 and CitWRKY1 could transactivate the promoter of CitAco3. Subcellular localization results showed that CitWRKY1 was located in the nucleus and CitNAC62 was not. Yeast two-hybrid analysis and bimolecular fluorescence complementation (BiFC) assays indicated that the two differently located transcription factors could interact with each other. Furthermore, BiFC showed that the protein-protein interaction occurred only in the nucleus, indicating the potential mobility of CitNAC62 in plant cells. A synergistic effect on citrate content was observed between CitNAC62 and CitWRKY1. Transient overexpression of CitNAC62 or CitWRKY1 led to significantly lower citrate content in citrus fruit. The combined expression of CitNAC62 and CitWRKY1 resulted in lower citrate content compared with the expression of CitNAC62 or CitWRKY1 alone. The transcript abundance of CitAco3 was consistent with the citrate content. Thus, we propose that a complex of CitWRKY1 and CitNAC62 contributes to citric acid degradation in citrus fruit, potentially via modulation of CitAco3
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Aconitase
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4 |
|a CitNAC62
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|a CitWRKY1
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|a citric acid
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|a protein–protein interaction
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|a transcriptional regulation
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 650 |
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|a Citric Acid
|2 NLM
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| 650 |
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7 |
|a 2968PHW8QP
|2 NLM
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| 700 |
1 |
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|a Yin, Xue-Ren
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Wen-Li
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Xiao-Fen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Bo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Kun-Song
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 13 vom: 15. Juni, Seite 3419-3426
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:68
|g year:2017
|g number:13
|g day:15
|g month:06
|g pages:3419-3426
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|u http://dx.doi.org/10.1093/jxb/erx187
|3 Volltext
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|d 68
|j 2017
|e 13
|b 15
|c 06
|h 3419-3426
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