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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.7b01069
|2 doi
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|a pubmed24n0909.xml
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|a (DE-627)NLM272933570
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|a (NLM)28614663
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Basit, H
|e verfasserin
|4 aut
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|a Electrochemically Triggered Release of Reagent to the Proximal Leaflet of a Microcavity Supported Lipid Bilayer
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.01.2019
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|a Date Revised 22.01.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A novel and versatile approach to electrichemically triggering the release of a reagent, β-cyclodextrin (β-CD), selectively to the proximal leaflet of a supported lipid bilayer is described. Selective delivery is achieved by creating a spanning lipid bilayer across a microcavity array and exploiting the irreversible redox disassembly of the host-guest complex formed between thiolated ferrocene (Fc) and β-cyclodextrin (β-CD) in the presence of chloride. Self-assembled monolayers of the ferrocene-alkanethiols were formed regioselectively on the interior surface of highly ordered 2.8 μm cavities while the exterior top surface of the array was blocked with a monolayer of mercaptoethanol. The Fc monolayers were complexed with β-CD or β-CD-conjugated to streptavidin (β-CD-SA). Phospholipid bilayers were then assembled across the array via combined Langmuir-Blodgett/vesicle fusion leading to a spanning bilayer suspended across the aqueous filled microcavities. Upon application of a positive potential, ferrocene is oxidized to ferrocinium cation, disrupting the inclusion complex and leading to the release of the β-CD into the microcavity solution where it diffuses to the lower leaflet of the suspended bilayer. Disassembly of the supramolecular complex within the cavities and binding of the β-CD-SA to a biotinylated bilayer was followed by voltammetry and impedance spectroscopy where it caused a large increase in membrane resistance. For unmodified β-CD, the extraction of cholesterol from a cholesterol containing bilayer was evident in a decrease in the bilayer resistance. For the first time, this direct approach to targeted delivery of a reagent to the proximal layer of a lipid bilayer offers the potential to build models of bidirectional signaling (inside-out vs outside-in) in cell membrane model systems
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|a Journal Article
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|a Lipid Bilayers
|2 NLM
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|a Phospholipids
|2 NLM
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|a Streptavidin
|2 NLM
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|a 9013-20-1
|2 NLM
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|a Cholesterol
|2 NLM
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|a 97C5T2UQ7J
|2 NLM
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|a Maher, S
|e verfasserin
|4 aut
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|a Forster, R J
|e verfasserin
|4 aut
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|a Keyes, T E
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 33(2017), 27 vom: 11. Juli, Seite 6691-6700
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:33
|g year:2017
|g number:27
|g day:11
|g month:07
|g pages:6691-6700
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|u http://dx.doi.org/10.1021/acs.langmuir.7b01069
|3 Volltext
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