Influence of Surface Charge of the Nanostructures on the Biocatalytic Activity

The physicochemical properties of abiotic nanostructures determine the structure and function of biological counterparts in biotic-abiotic nanohybrids. A comprehensive understanding of the interfacial interactions and the predictive capability of their structure and function is paramount for virtual...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 33(2017), 26 vom: 05. Juli, Seite 6611-6619
1. Verfasser: Tadepalli, Sirimuvva (VerfasserIn)
Weitere Verfasser: Wang, Zheyu, Liu, Keng-Ku, Jiang, Qisheng, Slocik, Joseph, Naik, Rajesh R, Singamaneni, Srikanth
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S.
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520 |a The physicochemical properties of abiotic nanostructures determine the structure and function of biological counterparts in biotic-abiotic nanohybrids. A comprehensive understanding of the interfacial interactions and the predictive capability of their structure and function is paramount for virtually all fields of bionanotechnology. In this study, using plasmonic nanostructures as a model abiotic system, we investigate the effect of the surface charge of nanostructures on the biocatalytic reaction kinetics of a bound enzyme. We found that the surface charge of nanostructures profoundly influences the structure, orientation, and activity of the bound enzyme. Furthermore, the interactions of the enzyme with nanoparticles result in stable conjugates that retain their functionality at elevated temperatures, unlike their free counterparts that lose their secondary structure and biocatalytic activity 
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650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
700 1 |a Wang, Zheyu  |e verfasserin  |4 aut 
700 1 |a Liu, Keng-Ku  |e verfasserin  |4 aut 
700 1 |a Jiang, Qisheng  |e verfasserin  |4 aut 
700 1 |a Slocik, Joseph  |e verfasserin  |4 aut 
700 1 |a Naik, Rajesh R  |e verfasserin  |4 aut 
700 1 |a Singamaneni, Srikanth  |e verfasserin  |4 aut 
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