Identification of Single Nucleotide Polymorphisms Associated with Brown Rust Resistance, α-Amylase Activity and Pre-harvest Sprouting in Rye (Secale cereale L.)

Rye is a crop with relatively high resistance to biotic and abiotic stresses. However, the resistance to brown rust (Puccinia recondita f. sp. secalis) and pre-harvest sprouting are still not satisfactory. High α-amylase activity is also among the main disadvantages of this species. Therefore, effec...

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Veröffentlicht in:Plant molecular biology reporter. - 1998. - 35(2017), 3 vom: 28., Seite 366-378
1. Verfasser: Rakoczy-Trojanowska, Monika (VerfasserIn)
Weitere Verfasser: Krajewski, Paweł, Bocianowski, Jan, Schollenberger, Małgorzata, Wakuliński, Wojciech, Milczarski, Paweł, Masojć, Piotr, Targońska-Karasek, Małgorzata, Banaszak, Zofia, Banaszak, Katarzyna, Brukwiński, Waldemar, Orczyk, Wacław, Kilian, Andrzej
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Plant molecular biology reporter
Schlagworte:Journal Article Candidate gene association mapping DArTSeq Genome-wide association mapping Kompetitive allele specific PCR ScBx genes
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520 |a Rye is a crop with relatively high resistance to biotic and abiotic stresses. However, the resistance to brown rust (Puccinia recondita f. sp. secalis) and pre-harvest sprouting are still not satisfactory. High α-amylase activity is also among the main disadvantages of this species. Therefore, effective tools, e.g. molecular markers, allowing precise and environmentally independent selection of favourable alleles are desirable. In the present study, two kinds of association mapping-genome-wide association mapping (GWAM) based on sequences of DArTSeq markers and candidate gene association mapping (CGAM) based on sequences of ScBx genes-were chosen for development of molecular markers fulfilling these criteria. The analysed population consisted of 149 diverse inbred lines (DILs). Altogether, 67 and 11 single nucleotide polymorphisms (SNPs) identified in, respectively, GWAM and CGAM, were significantly associated with the investigated traits: 2 SNPs with resistance to brown rust, 71 SNPs with resistance to pre-harvest sprouting and 5 SNPs with α-amylase activity in the grain. Fifteen SNPs were stable across all environments. The highest number (13) of environmentally stable SNPs was associated with pre-harvest sprouting resistance. The test employing the Kompetitive Allele Specific PCR method proved the versatility of four markers identified in both GWAM and CGAM 
650 4 |a Journal Article 
650 4 |a Candidate gene association mapping 
650 4 |a DArTSeq 
650 4 |a Genome-wide association mapping 
650 4 |a Kompetitive allele specific PCR 
650 4 |a ScBx genes 
700 1 |a Krajewski, Paweł  |e verfasserin  |4 aut 
700 1 |a Bocianowski, Jan  |e verfasserin  |4 aut 
700 1 |a Schollenberger, Małgorzata  |e verfasserin  |4 aut 
700 1 |a Wakuliński, Wojciech  |e verfasserin  |4 aut 
700 1 |a Milczarski, Paweł  |e verfasserin  |4 aut 
700 1 |a Masojć, Piotr  |e verfasserin  |4 aut 
700 1 |a Targońska-Karasek, Małgorzata  |e verfasserin  |4 aut 
700 1 |a Banaszak, Zofia  |e verfasserin  |4 aut 
700 1 |a Banaszak, Katarzyna  |e verfasserin  |4 aut 
700 1 |a Brukwiński, Waldemar  |e verfasserin  |4 aut 
700 1 |a Orczyk, Wacław  |e verfasserin  |4 aut 
700 1 |a Kilian, Andrzej  |e verfasserin  |4 aut 
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