Real-time monitoring of methane oxidation in a simulated landfill cover soil and MiSeq pyrosequencing analysis of the related bacterial community structure
Copyright © 2017 Elsevier Ltd. All rights reserved.
| Publié dans: | Waste management (New York, N.Y.). - 1999. - 68(2017) vom: 15. Okt., Seite 369-377 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2017
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| Accès à la collection: | Waste management (New York, N.Y.) |
| Sujets: | Journal Article Bacterial community structure CH(4) oxidation kinetics Methanotrophs Real time on line monitoring Simulated landfill cover soil Soil Methane OP0UW79H66 |
| Résumé: | Copyright © 2017 Elsevier Ltd. All rights reserved. Real-time CH4 oxidation in a landfill cover soil was studied using automated gas sampling that determined biogas (CH4 and CO2) and O2 concentrations at various depths in a simulated landfill cover soil (SLCS) column reactor. The real-time monitoring system obtained more than 10,000 biogas (CH4 and CO2) and O2 data points covering 32 steady states of CH4 oxidation with 32 different CH4 fluxes (0.2-125mol·m-2·d-1). The kinetics of CH4 oxidation at different depths (0-20cm, 20-40cm, and 40-60cm) of SLCS were well fit by a CH4-O2 dual-substrate model based on 32 values (averaged, n=5-15) of equilibrated CH4 concentrations. The quality of the fit (R2 ranged from 0.90 to 0.96) was higher than those reported in previous studies, which suggests that real time monitoring is beneficial for CH4 oxidation simulations. MiSeq pyrosequencing indicated that CH4 flux events changed the bacterial community structure (e.g., increased the abundance of Bacteroidetes and Methanotrophs) and resulted in a relative increase in the amount of type I methanotrophs (Methylobacter and Methylococcales) and a decrease in the amount of type II methanotrophs (Methylocystis) |
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| Description: | Date Completed 13.12.2017 Date Revised 02.12.2018 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1879-2456 |
| DOI: | 10.1016/j.wasman.2017.05.007 |