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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erx162
|2 doi
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|a pubmed24n1223.xml
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|a (DE-627)NLM27213421X
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|a (NLM)28531251
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Costa, Alex
|e verfasserin
|4 aut
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| 245 |
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|a Ca2+-dependent phosphoregulation of the plasma membrane Ca2+-ATPase ACA8 modulates stimulus-induced calcium signatures
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.02.2018
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|a Date Revised 13.12.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Ca2+ signals are transient, hence, upon a stimulus-induced increase in cytosolic Ca2+ concentration, cells have to re-establish resting Ca2+ levels. Ca2+ extrusion is operated by a wealth of transporters, such as Ca2+ pumps and Ca2+/H+ antiporters, which often require a rise in Ca2+ concentration to be activated. Here, we report a regulatory fine-tuning mechanism of the Arabidopsis thaliana plasma membrane-localized Ca2+-ATPase isoform ACA8 that is mediated by calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) complexes. We show that two CIPKs (CIPK9 and CIPK14) are able to interact with ACA8 in vivo and phosphorylate it in vitro. Transient co-overexpression of ACA8 with CIPK9 and the plasma membrane Ca2+ sensor CBL1 in tobacco leaf cells influences nuclear Ca2+ dynamics, specifically reducing the height of the second peak of the wound-induced Ca2+ transient. Stimulus-induced Ca2+ transients in mature leaves and seedlings of an aca8 T-DNA insertion line exhibit altered dynamics when compared with the wild type. Altogether our results identify ACA8 as a prominent in vivo regulator of cellular Ca2+ dynamics and reveal the existence of a Ca2+-dependent CBL-CIPK-mediated regulatory feedback mechanism, which crucially functions in the termination of Ca2+ signals
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|a Journal Article
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|a Arabidopsis thaliana
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|a CBL-interacting protein kinases
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|a Ca2+ signature
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|a calcineurin B-like protein
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|a phosphorylation
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|a plasma membrane Ca2+-ATPase
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|a Arabidopsis Proteins
|2 NLM
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|a Aca8 protein, Arabidopsis
|2 NLM
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|a EC 7.2.2.10
|2 NLM
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| 650 |
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|a Calcium-Transporting ATPases
|2 NLM
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| 650 |
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|a EC 7.2.2.10
|2 NLM
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| 650 |
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|a Calcium
|2 NLM
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| 650 |
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|a SY7Q814VUP
|2 NLM
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| 700 |
1 |
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|a Luoni, Laura
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Marrano, Claudia Adriana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hashimoto, Kenji
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Köster, Philipp
|e verfasserin
|4 aut
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| 700 |
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|a Giacometti, Sonia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a De Michelis, Maria Ida
|e verfasserin
|4 aut
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| 700 |
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|a Kudla, Jörg
|e verfasserin
|4 aut
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| 700 |
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|a Bonza, Maria Cristina
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 12 vom: 01. Juni, Seite 3215-3230
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:68
|g year:2017
|g number:12
|g day:01
|g month:06
|g pages:3215-3230
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|u http://dx.doi.org/10.1093/jxb/erx162
|3 Volltext
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|d 68
|j 2017
|e 12
|b 01
|c 06
|h 3215-3230
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