|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM272036811 |
| 003 |
DE-627 |
| 005 |
20250221162128.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erx121
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0906.xml
|
| 035 |
|
|
|a (DE-627)NLM272036811
|
| 035 |
|
|
|a (NLM)28520898
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Mitchell, Madeline C
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Pyrenoid loss impairs carbon-concentrating mechanism induction and alters primary metabolism in Chlamydomonas reinhardtii
|
| 264 |
|
1 |
|c 2017
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 14.05.2018
|
| 500 |
|
|
|a Date Revised 13.11.2018
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a Carbon-concentrating mechanisms (CCMs) enable efficient photosynthesis and growth in CO2-limiting environments, and in eukaryotic microalgae localisation of Rubisco to a microcompartment called the pyrenoid is key. In the model green alga Chlamydomonas reinhardtii, Rubisco preferentially relocalises to the pyrenoid during CCM induction and pyrenoid-less mutants lack a functioning CCM and grow very poorly at low CO2. The aim of this study was to investigate the CO2 response of pyrenoid-positive (pyr+) and pyrenoid-negative (pyr-) mutant strains to determine the effect of pyrenoid absence on CCM induction and gene expression. Shotgun proteomic analysis of low-CO2-adapted strains showed reduced accumulation of some CCM-related proteins, suggesting that pyr- has limited capacity to respond to low-CO2 conditions. Comparisons between gene transcription and protein expression revealed potential regulatory interactions, since Rubisco protein linker (EPYC1) protein did not accumulate in pyr- despite increased transcription, while elements of the LCIB/LCIC complex were also differentially expressed. Furthermore, pyr- showed altered abundance of a number of proteins involved in primary metabolism, perhaps due to the failure to adapt to low CO2. This work highlights two-way regulation between CCM induction and pyrenoid formation, and provides novel candidates for future studies of pyrenoid assembly and CCM function
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Carbon-concentrating mechanism
|
| 650 |
|
4 |
|a Chlamydomonas
|
| 650 |
|
4 |
|a photosynthesis
|
| 650 |
|
4 |
|a proteomics
|
| 650 |
|
4 |
|a pyrenoid
|
| 650 |
|
4 |
|a tandem mass spectrometry
|
| 650 |
|
7 |
|a Algal Proteins
|2 NLM
|
| 650 |
|
7 |
|a Carbon
|2 NLM
|
| 650 |
|
7 |
|a 7440-44-0
|2 NLM
|
| 700 |
1 |
|
|a Metodieva, Gergana
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Metodiev, Metodi V
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Griffiths, Howard
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Meyer, Moritz T
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 14 vom: 01. Juni, Seite 3891-3902
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:68
|g year:2017
|g number:14
|g day:01
|g month:06
|g pages:3891-3902
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erx121
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 68
|j 2017
|e 14
|b 01
|c 06
|h 3891-3902
|