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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erx146
|2 doi
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|a pubmed24n0906.xml
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|a (DE-627)NLM271901985
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|a (NLM)28505379
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Brenner, Wolfram G
|e verfasserin
|4 aut
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| 245 |
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|a Analysis of CFB, a cytokinin-responsive gene of Arabidopsis thaliana encoding a novel F-box protein regulating sterol biosynthesis
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 29.11.2017
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|a Date Revised 24.01.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Protein degradation by the ubiquitin-26S proteasome pathway is important for the regulation of cellular processes, but the function of most F-box proteins relevant to substrate recognition is unknown. We describe the analysis of the gene Cytokinin-induced F-box encoding (CFB, AT3G44326), identified in a meta-analysis of cytokinin-related transcriptome studies as one of the most robust cytokinin response genes. F-box domain-dependent interaction with the E3 ubiquitin ligase complex component ASK1 classifies CFB as a functional F-box protein. Apart from F-box and transmembrane domains, CFB contains no known functional domains. CFB is expressed in all plant tissues, predominantly in root tissue. A ProCFB:GFP-GUS fusion gene showed strongest expression in the lateral root cap and during lateral root formation. CFB-GFP fusion proteins were mainly localized in the nucleus and the cytosol but also at the plasma membrane. cfb mutants had no discernible phenotype, but CFB overexpressing plants showed several defects, such as a white upper inflorescence stem, similar to the hypomorphic cycloartenol synthase mutant cas1-1. Both CFB overexpressing plants and cas1-1 mutants accumulated the CAS1 substrate 2,3-oxidosqualene in the white stem tissue, the latter even more after cytokinin treatment, indicating impairment of CAS1 function. This suggests that CFB may link cytokinin and the sterol biosynthesis pathway
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|a Journal Article
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|a Albinism
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|a F-box protein
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|a chloroplast
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|a cycloartenol synthase
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|a cytokinin
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|a plastid
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|a sterol biosynthesis
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|a ASK1 protein, Arabidopsis
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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|a CFB protein, Arabidopsis
|2 NLM
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|a Cytokinins
|2 NLM
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| 650 |
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|a DNA, Bacterial
|2 NLM
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| 650 |
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|a F-Box Proteins
|2 NLM
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| 650 |
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|a Phytosterols
|2 NLM
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| 650 |
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|a Recombinant Fusion Proteins
|2 NLM
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| 650 |
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|a T-DNA
|2 NLM
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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| 650 |
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|a 147336-22-9
|2 NLM
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| 650 |
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|a Ubiquitin-Protein Ligases
|2 NLM
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| 650 |
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|a EC 2.3.2.27
|2 NLM
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| 650 |
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|a Intramolecular Transferases
|2 NLM
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| 650 |
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|a EC 5.4.-
|2 NLM
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|a cycloartenol synthase
|2 NLM
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| 650 |
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|a EC 5.4.99.8
|2 NLM
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| 700 |
1 |
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|a Leuendorf, Jan Erik
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cortleven, Anne
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martin, Laetitia B B
|e verfasserin
|4 aut
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| 700 |
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|a Schaller, Hubert
|e verfasserin
|4 aut
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| 700 |
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|a Schmülling, Thomas
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 11 vom: 17. Mai, Seite 2769-2785
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:68
|g year:2017
|g number:11
|g day:17
|g month:05
|g pages:2769-2785
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erx146
|3 Volltext
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|a AR
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|d 68
|j 2017
|e 11
|b 17
|c 05
|h 2769-2785
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