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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erx165
|2 doi
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|a pubmed25n0906.xml
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|a (DE-627)NLM271901713
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|a (NLM)28505352
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Chen, Yi
|e verfasserin
|4 aut
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| 245 |
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|a The Nodulin 26-like intrinsic membrane protein OsNIP3;2 is involved in arsenite uptake by lateral roots in rice
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|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 29.11.2017
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|a Date Revised 06.10.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Previous studies have shown that the Nodulin 26-like intrinsic membrane protein (NIP) Lsi1 (OsNIP2;1) is involved in arsenite [As(III)] uptake in rice (Oryza sativa). However, the role of other rice NIPs in As(III) accumulation in planta remains unknown. In the present study, we investigated the role OsNIP3;2 in As(III) uptake in rice. When expressed in Xenopus laevis oocytes, OsNIP3;2 showed a high transport activity for As(III). Quantitative real-time RT-PCR showed that the expression of OsNIP3;2 was suppressed by 5 µM As(III), but enhanced by 20 and 100 µM As(III). Transgenic rice plants expressing OsNIP3;2pro-GUS showed that the gene was predominantly expressed in the lateral roots and the stele region of the primary roots. Transient expression of OsNIP3;2:GFP fusion protein in rice protoplasts showed that the protein was localized in the plasma membrane. Knockout of OsNIP3;2 significantly decreased As concentration in the roots, but had little effect on shoot As concentration. Synchrotron microfocus X-ray fluorescence showed decreased As accumulation in the stele of the lateral roots in the mutants compared with wild-type. Our results indicate that OsNIP3;2 is involved in As(III) uptake by lateral roots, but its contribution to As accumulation in the shoots is limited
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arsenic
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|a Nodulin 26-like intrinsic membrane proteins (NIPs)
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|a arsenite
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|a lateral root
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|a rice
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|a uptake
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|a Arsenites
|2 NLM
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| 650 |
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|a Membrane Proteins
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Recombinant Proteins
|2 NLM
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| 650 |
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|a nodulin
|2 NLM
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| 650 |
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|a Silicic Acid
|2 NLM
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| 650 |
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|a 1343-98-2
|2 NLM
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| 650 |
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|a arsenite
|2 NLM
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| 650 |
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|a N5509X556J
|2 NLM
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| 700 |
1 |
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|a Sun, Sheng-Kai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tang, Zhong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Guidong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Moore, Katie L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Maathuis, Frans J M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Miller, Antony J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a McGrath, Steve P
|e verfasserin
|4 aut
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| 700 |
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|a Zhao, Fang-Jie
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 11 vom: 17. Mai, Seite 3007-3016
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:68
|g year:2017
|g number:11
|g day:17
|g month:05
|g pages:3007-3016
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|u http://dx.doi.org/10.1093/jxb/erx165
|3 Volltext
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|a AR
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|d 68
|j 2017
|e 11
|b 17
|c 05
|h 3007-3016
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