Identification of factors required for m6 A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI
© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.
| Veröffentlicht in: | The New phytologist. - 1979. - 215(2017), 1 vom: 15. Juli, Seite 157-172 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2017
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Arabidopsis HAKAI N6-adenosine methylation (m6A) VIRILIZER mRNA methylation protoxylem Arabidopsis Proteins RNA, Messenger 6-methyladenine mRNA methyltransferase mehr... |
| Zusammenfassung: | © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust. N6-adenosine methylation (m6 A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m6 A writer proteins in Arabidopsis thaliana. The components required for m6 A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m6 A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m6 A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m6 A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences |
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| Beschreibung: | Date Completed 01.05.2018 Date Revised 29.01.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.14586 |