Fabrication of Photoreactive Biocomposite Coatings via Electric Field-Assisted Assembly of Cyanobacteria
We report how dielectrophoresis (DEP) can be used as a tool for the fabrication of biocomposite coatings of photoreactive cyanobacteria (Synechococcus PCC7002) on flexible polyester sheets (PEs). The PE substrates were precoated by a layer-by-layer assembled film of charged polyelectrolytes. In exce...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 33(2017), 21 vom: 30. Mai, Seite 5304-5313 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2017
|
| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Biocompatible Materials Polyesters |
| Zusammenfassung: | We report how dielectrophoresis (DEP) can be used as a tool for the fabrication of biocomposite coatings of photoreactive cyanobacteria (Synechococcus PCC7002) on flexible polyester sheets (PEs). The PE substrates were precoated by a layer-by-layer assembled film of charged polyelectrolytes. In excellent agreement between experimental data and numerical simulations, the directed assembly process driven by external electric field results in the formation of 1D chains and 2D sheets by the cells. The preassembled cyanobacteria chains and arrays became deposited on the substrate and remained in place after the electric field was turned off due to the electrostatic attraction between the negatively charged cell surfaces and the positively charged polyelectrolyte-coated PE. The DEP-assisted packing of cyanobacteria is close to the maximal surface coverage of ∼70% estimated from convectively assembled monolayers. Confocal laser scanning microscopy and spectrophotometry confirm that the photosynthetic pigment integrity of the Synechococcus cells is preserved after DEP immobilization. The significant decrease of the light scattering and the enhanced transmittance of these field-assembled cyanobacteria coatings demonstrate reduced self-shading compared to suspension cultures. Thus, we achieved the assembly of structured cyanobacteria coatings that optimize cell surface coverage and preserve cell viability after immobilization. This is a step toward the development of flexible multilayered cell-based photoabsorbing biomaterials that can serve as components of "biomimetic leaves" for utilizing solar energy to recycle CO2 into fuels or chemicals |
|---|---|
| Beschreibung: | Date Completed 23.01.2019 Date Revised 23.01.2019 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/acs.langmuir.7b00335 |