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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1111/nph.14563
|2 doi
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|a pubmed25n0904.xml
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|a (DE-627)NLM271380330
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|a (NLM)28452060
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wei, Hongbin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a CiMYB17, a stress-induced chicory R2R3-MYB transcription factor, activates promoters of genes involved in fructan synthesis and degradation
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.05.2018
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|a Date Revised 09.01.2024
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|a published: Print-Electronic
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|a GENBANK: KY385878, AY323935.1, KY385874, KY385875, KY38586, KY385877, EU545648.1, EU545647.1, KY385879, KY385871, KY385872, KY385873, KY354369, KY354370, JF288934
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|a Citation Status MEDLINE
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|a © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.
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|a In Cichorium intybus, inulin metabolism is mediated by fructan-active enzymes (FAZYs): sucrose:sucrose 1-fructosyltransferase (1-SST), fructan:fructan 1-fructosyltransferase (1-FFT), and fructan 1-exohydrolases 1, 2a and 2b (1-FEH1, -2a and -2b), respectively. While these enzymes have been rigorously characterized, the transcriptional network orchestrating their development- and stress-related expression has remained largely unknown. Here, the possible role of R2R3-MYB transcription factors in FAZY regulation was explored via bioinformatic identification of R2R3-MYBs (using an RNA sequencing (RNAseq) database), studies of co-expression of these factors with target genes, in vivo transient transactivation assays of FAZY target promoters (dual luciferase assay), and a yeast one-hybrid assay investigating the specificity of the binding of these factors to cis-elements. The chicory MYB transcription factor CiMYB17 specifically activated promoters of 1-SST and 1-FFT by binding to the consensus DNA-motif DTTHGGT. Unexpectedly, CiMYB17 also activated promoters of fructan exohydrolase genes. The stimulatory effect on promoter activities of sucrose transporter and cell wall invertase genes points to a general role in regulating the source-sink relationship. Co-induction of CiMYB17 with 1-SST and 1-FFT (and, less consistently, with 1-FEH1/2) in nitrogen-starved or abscisic acid (ABA)-treated chicory seedlings and in salt-stressed chicory hairy roots supports a role in stress-induced fructan metabolism, including de novo fructan synthesis and trimming of pre-existing fructans, whereas the reduced expression of CiMYB17 in developing taproots excludes a role in fructan accumulation under normal growth conditions
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|a Journal Article
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|a cell wall invertase
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|a fructan active enzymes (FAZYs)
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|a myeloblastosis (MYB) transcription factor
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| 650 |
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4 |
|a source-sink relationship
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| 650 |
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|a sucrose transporters
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| 650 |
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|a Fructans
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 700 |
1 |
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|a Bausewein, Anja
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Greiner, Steffen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dauchot, Nicolas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Harms, Karsten
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rausch, Thomas
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1990
|g 215(2017), 1 vom: 01. Juli, Seite 281-298
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:215
|g year:2017
|g number:1
|g day:01
|g month:07
|g pages:281-298
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|u http://dx.doi.org/10.1111/nph.14563
|3 Volltext
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|a AR
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|d 215
|j 2017
|e 1
|b 01
|c 07
|h 281-298
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