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231224s2018 xx |||||o 00| ||eng c |
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|a 10.5713/ajas.17.0153
|2 doi
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|a pubmed25n0903.xml
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|a (DE-627)NLM271138009
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|a (NLM)28427254
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|a DE-627
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|a eng
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|a Bae, Gui-Seck
|e verfasserin
|4 aut
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|a Supplementing Rhodobacter sphaeroides in the diet of lactating Holstein cows may naturally produce coenzyme Q10-enriched milk
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 01.10.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a OBJECTIVE: To examine the effects of Rhodobacter sphaeroides (R. sphaeroides) supplementation as a direct-fed microbial (DFM) on rumen fermentation in dairy cows and on coenzyme Q10 (CoQ10) transition into milk, an in vitro rumen simulation batch culture and an in vivo dairy cow experiment were conducted
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|a METHODS: The characteristics of in vitro ruminal fermentation were investigated using rumen fluids from six cannulated Holstein dairy cows at 2 h post-afternoon feeding. A control treatment was included in the experiments based on a typified total mixed ration (TMR) for lactating dairy cows, which was identical to the one used in the in vivo study, plus R. sphaeroides at 0.1%, 0.3%, and 0.5% TMR dry matter. The in vivo study employed six ruminally cannulated lactating Holstein cows randomly allotted to either the control TMR (C-TMR) treatment or to a diet supplemented with a 0.5% R. sphaeroides culture (S-TMR, dry matter basis) ad libitum. The presence of R. sphaeroides was verified using denaturing gradient gel electrophoresis (DGGE) applied to the bacterial samples obtained from the in vivo study. The concentration of CoQ10 in milk and in the supernatant from the in vitro study was determined using high performance liquid chromatography
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|a RESULTS: The results of the in vitro batch culture and DGGE showed that the concentration of CoQ10 significantly increased after 2 h of R. sphaeroides supplementation above 0.1%. When supplemented to the diet of lactating cows at the level of 0.5%, R. sphaeroides did not present any adverse effect on dry matter intake and milk yield. However, the concentration of CoQ10 in milk dramatically increased, with treated cows producing 70.9% more CoQ10 than control cows
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|a CONCLUSION: The CoQ10 concentration in milk increased via the use of a novel DFM, and R. sphaeroides might be used for producing value-added milk and dairy products in the future
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|a Journal Article
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|a Coenzyme Q10
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|a Dairy Cow
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|a Denaturing Gradient Gel Electrophoresis (DGGE)
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|a In vitro
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|a Rhodobacter sphaeroides
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1 |
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|a Choi, Ahreum
|e verfasserin
|4 aut
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|a Yeo, Joon Mo
|e verfasserin
|4 aut
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|a Kim, Jong Nam
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Song, Jaeyong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, Eun Joong
|e verfasserin
|4 aut
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|a Chang, Moon Baek
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Asian-Australasian journal of animal sciences
|d 1998
|g 31(2018), 1 vom: 11. Jan., Seite 40-46
|w (DE-627)NLM098195883
|x 1011-2367
|7 nnns
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| 773 |
1 |
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|g volume:31
|g year:2018
|g number:1
|g day:11
|g month:01
|g pages:40-46
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|u http://dx.doi.org/10.5713/ajas.17.0153
|3 Volltext
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