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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.12618
|2 doi
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|a pubmed24n0903.xml
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|a eng
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| 100 |
1 |
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|a Reichert, M
|e verfasserin
|4 aut
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| 245 |
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|a Antiviral activity of exopolysaccharides from Arthrospira platensis against koi herpesvirus
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 14.05.2018
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2017 John Wiley & Sons Ltd.
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|a Although koi herpesvirus (KHV) has a history of causing severe economic losses in common carp and koi farms, there are still no treatments available on the market. Thus, the aim of this study was to test exopolysaccharides (EPS) for its antiviral activity against KHV, by monitoring inhibition and cytotoxic effects in common carp brain cells. These substances can be easily extracted from extracellular algae supernatant and were identified as groups of sulphated polysaccharides. In order to reach this aim, Arthrospira platensis, which is well known for its antiviral activity of intra- and extracellular compounds towards mammalian herpesviruses, was investigated as standard organism and compared to commercial antiviral drug, ganciclovir, which inhibits the viral DNA polymerization. The antiviral activity of polysaccharides of A. platensis against KHV was confirmed in vitro using qualitative assessment of KHV life cycle genes, and it was found by RT-PCR that EPS, applied at a concentration of >18 μg mL-1 and a multiplicity of infection (MOI) of 0.45 of KHV, suppressed the viral replication in common carp brain (CCB) cells even after 22 days post-infection, entirely. Further, this study presents first data indicating an enormous potential using polysaccharides as an additive for aquacultures to lower or hinder the spread of the KHV and koi herpesvirus disease (KHVD) in future
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|a Journal Article
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|a KHVD
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|a antiviral substances
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|a exopolysaccharides
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|a koi herpesvirus
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| 650 |
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|a Antiviral Agents
|2 NLM
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|a Polysaccharides, Bacterial
|2 NLM
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| 700 |
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|a Bergmann, S M
|e verfasserin
|4 aut
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|a Hwang, J
|e verfasserin
|4 aut
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|a Buchholz, R
|e verfasserin
|4 aut
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| 700 |
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|a Lindenberger, C
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 40(2017), 10 vom: 15. Okt., Seite 1441-1450
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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|g volume:40
|g year:2017
|g number:10
|g day:15
|g month:10
|g pages:1441-1450
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|u http://dx.doi.org/10.1111/jfd.12618
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