|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM271062142 |
| 003 |
DE-627 |
| 005 |
20231224231909.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erx117
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0903.xml
|
| 035 |
|
|
|a (DE-627)NLM271062142
|
| 035 |
|
|
|a (NLM)28419381
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Cooper, James W
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Enhancing faba bean (Vicia faba L.) genome resources
|
| 264 |
|
1 |
|c 2017
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 30.01.2018
|
| 500 |
|
|
|a Date Revised 13.11.2018
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a Grain legume improvement is currently impeded by a lack of genomic resources. The paucity of genome information for faba bean can be attributed to the intrinsic difficulties of assembling/annotating its giant (~13 Gb) genome. In order to address this challenge, RNA-sequencing analysis was performed on faba bean (cv. Wizard) leaves. Read alignment to the faba bean reference transcriptome identified 16 300 high quality unigenes. In addition, Illumina paired-end sequencing was used to establish a baseline for genomic information assembly. Genomic reads were assembled de novo into contigs with a size range of 50-5000 bp. Over 85% of sequences did not align to known genes, of which ~10% could be aligned to known repetitive genetic elements. Over 26 000 of the reference transcriptome unigenes could be aligned to DNA-sequencing (DNA-seq) reads with high confidence. Moreover, this comparison identified 56 668 potential splice points in all identified unigenes. Sequence length data were extended at 461 putative loci through alignment of DNA-seq contigs to full-length, publicly available linkage marker sequences. Reads also yielded coverages of 3466× and 650× for the chloroplast and mitochondrial genomes, respectively. Inter- and intraspecies organelle genome comparisons established core legume organelle gene sets, and revealed polymorphic regions of faba bean organelle genomes
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Illumina sequencing
|
| 650 |
|
4 |
|a RNA-seq analysis.
|
| 650 |
|
4 |
|a legumes
|
| 650 |
|
4 |
|a mitochondrial genome
|
| 650 |
|
4 |
|a plastome
|
| 650 |
|
4 |
|a protein security
|
| 700 |
1 |
|
|a Wilson, Michael H
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Derks, Martijn F L
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Smit, Sandra
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kunert, Karl J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Cullis, Christopher
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Foyer, Christine H
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 8 vom: 01. Apr., Seite 1941-1953
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:68
|g year:2017
|g number:8
|g day:01
|g month:04
|g pages:1941-1953
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erx117
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 68
|j 2017
|e 8
|b 01
|c 04
|h 1941-1953
|