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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2017.03.014
|2 doi
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|a pubmed25n0903.xml
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|a (DE-627)NLM270986642
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|a (NLM)28411489
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|a (PII)S0176-1617(17)30079-2
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|a DE-627
|b ger
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Da-Hong
|e verfasserin
|4 aut
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|a Kale BoRACK1 is involved in the plant response to salt stress and Peronospora brassicae Gaumann
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.11.2017
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2017 Elsevier GmbH. All rights reserved.
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|a The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, a kale (Brassica oleracea var. acephala f.tricolor) RACK1 gene (BoRACK1) was cloned by RT-PCR. The amino acid sequence of BoRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides. Comparison with AtRACK1 from Arabidopsis revealed 87.1% identity at the amino acid level. Expression pattern analysis by RT-PCR showed that BoRACK1 was expressed in all analyzed tissues of kale and that its transcription in leaves was down-regulated by salt, abscisic acid, and H2O2 at a high concentration. Overexpression of BoRACK1 in kale led to a reduction in symptoms caused by Peronospora brassicae Gaumann on kale leaves. The expression levels of the pathogenesis-related protein genes, PR-1 and PRB-1, increased 2.5-4-fold in transgenic kale, and reactive oxygen species production was more active than in the wild-type. They also exhibited increased tolerance to salt stress in seed germination. H2O2 may also be involved in the regulation of BoRACK1 during seed germination under salt stress. Quantitative real-time PCR analyses showed that the transcript levels of BoRbohs genes were significantly higher in overexpression of BoRACK1 transgenic lines. Yeast two-hybrid assays showed that BoRACK1 could interact with WNK8, eIF6, RAR1, and SGT1. This study and previous work lead us to believe that BoRACK1 may form a complex with regulators of plant salt and disease resistance to coordinate kale reactions to pathogens
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|a Journal Article
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|a Brassica oleracea
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|a Disease resistance
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|a RACK1
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|a Salt stress
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|a Plant Proteins
|2 NLM
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|a Sodium Chloride
|2 NLM
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|a 451W47IQ8X
|2 NLM
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|a Hydrogen Peroxide
|2 NLM
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|a BBX060AN9V
|2 NLM
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| 700 |
1 |
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|a Shen, Fu-Jia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Hong-Yan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Wei
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 213(2017) vom: 01. Juni, Seite 188-198
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:213
|g year:2017
|g day:01
|g month:06
|g pages:188-198
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|u http://dx.doi.org/10.1016/j.jplph.2017.03.014
|3 Volltext
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