Transient dominant host-range selection using Chinese hamster ovary cells to generate marker-free recombinant viral vectors from vaccinia virus

Recombinant vaccinia viruses (rVACVs) are promising antigen-delivery systems for vaccine development that are also useful as research tools. Two common methods for selection during construction of rVACV clones are (i) co-insertion of drug resistance or reporter protein genes, which requires the use...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:	BioTechniques. - 1993. - 62(2017), 4 vom: 01. Apr., Seite 183-187
1. Verfasser:	Liu, Liang (VerfasserIn)
Weitere Verfasser:	Cooper, Tamara, Eldi, Preethi, Garcia-Valtanen, Pablo, Diener, Kerrilyn R, Howley, Paul M, Hayball, John D
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2017
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't CHO CP77 Vaccinia host-range factor recombinant virus construction

Beschreibung
Zusammenfassung:	Recombinant vaccinia viruses (rVACVs) are promising antigen-delivery systems for vaccine development that are also useful as research tools. Two common methods for selection during construction of rVACV clones are (i) co-insertion of drug resistance or reporter protein genes, which requires the use of additional selection drugs or detection methods, and (ii) dominant host-range selection. The latter uses VACV variants rendered replication-incompetent in host cell lines by the deletion of host-range genes. Replicative ability is restored by co-insertion of the host-range genes, providing for dominant selection of the recombinant viruses. Here, we describe a new method for the construction of rVACVs using the cowpox CP77 protein and unmodified VACV as the starting material. Our selection system will expand the range of tools available for positive selection of rVACV during vector construction, and it is substantially more high-fidelity than approaches based on selection for drug resistance
Beschreibung:	Date Completed 28.04.2017 Date Revised 06.03.2018 published: Electronic Citation Status MEDLINE
ISSN:	1940-9818
DOI:	10.2144/000114537