Arabidopsis MAP65-4 plays a role in phragmoplast microtubule organization and marks the cortical cell division site

© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 215(2017), 1 vom: 15. Juli, Seite 187-201
1. Verfasser: Li, Haoge (VerfasserIn)
Weitere Verfasser: Sun, Baojuan, Sasabe, Michiko, Deng, Xingguang, Machida, Yasunori, Lin, Honghui, Julie Lee, Y-R, Liu, Bo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Arabidopsis thaliana cell division site cytokinesis microtubule-associated protein microtubules (MTs) phragmoplast Arabidopsis Proteins MAP65-4 protein, Arabidopsis Microtubule-Associated Proteins
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520 |a The evolutionarily conserved MAP65 family proteins bundle anti-parallel microtubules (MTs). In Arabidopsis thaliana, mutations in the MAP65-3 gene lead to serious defects in MT organization in the phragmoplast and cause failures in cytokinesis. However, the functions of other ArabidopsisMAP65 isoforms are largely unknown. MAP65 functions were analyzed based on genetic interactions among different map65 mutations. Live-cell imaging and immunolocalization experiments revealed dynamic activities of two closely related MAP65 proteins in dividing cells. The map65-4 mutation caused synthetic lethality with map65-3 although map65-4 alone did not cause a noticeable phenotype. Furthermore, the introduction of an extra copy of the MAP65-4 gene significantly suppressed defects in cytokinesis and seedling growth caused by map65-3 because of restoring MT engagement in the spindle midzone. During mitosis, MAP65-4 first appeared at the preprophase band and persisted at the cortical division site afterwards. It was also concentrated on MTs in the spindle midzone and the phragmoplast. In the absence of MAP65-3, MAP65-4 exhibited greatly enhanced localization in the midzone of developing phragmoplast. Therefore, we have uncovered redundant but differential contributions of MAP65-3 and MAP65-4 to engaging and bundling anti-parallel MTs in the phragmoplast and disclosed a novel action of MAP65-4 at the cortical cell division site 
650 4 |a Journal Article 
650 4 |a Arabidopsis thaliana 
650 4 |a cell division site 
650 4 |a cytokinesis 
650 4 |a microtubule-associated protein 
650 4 |a microtubules (MTs) 
650 4 |a phragmoplast 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a MAP65-4 protein, Arabidopsis  |2 NLM 
650 7 |a Microtubule-Associated Proteins  |2 NLM 
700 1 |a Sun, Baojuan  |e verfasserin  |4 aut 
700 1 |a Sasabe, Michiko  |e verfasserin  |4 aut 
700 1 |a Deng, Xingguang  |e verfasserin  |4 aut 
700 1 |a Machida, Yasunori  |e verfasserin  |4 aut 
700 1 |a Lin, Honghui  |e verfasserin  |4 aut 
700 1 |a Julie Lee, Y-R  |e verfasserin  |4 aut 
700 1 |a Liu, Bo  |e verfasserin  |4 aut 
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773 1 8 |g volume:215  |g year:2017  |g number:1  |g day:15  |g month:07  |g pages:187-201 
856 4 0 |u http://dx.doi.org/10.1111/nph.14532  |3 Volltext 
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