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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.7b00314
|2 doi
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|a pubmed25n0901.xml
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|a DE-627
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|a eng
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| 100 |
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|a Wong, Kerwin K W
|e verfasserin
|4 aut
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|a Role of Cell Appendages in Initial Attachment and Stability of E. coli on Silica Monitored by Nondestructive TIRF Microscopy
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 20.06.2018
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|a Date Revised 20.06.2018
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Total internal reflection fluorescence (TIRF) microscopy was used to investigate initial attachment and stability of wild-type, curli-deficient (ΔcsgA), flagella-deficient (ΔflhDC), and type-1 fimbriae-deficient (Δfim) mutant E. coli strains. Suspended bacteria were injected into a flow cell where they deposited on a silica coverslip, and images were acquired over a 2 min period. TIRF microscope image analysis revealed that curli- and flagella-deficient mutants attached closer to the surface and required a longer time to find their equilibrium position (i.e., bond maturation) as compared to the wild-type and fimbriae-deficient mutants. Analysis of the change in bacterial surface area over the 2 min period also indicated that curli- and flagella-deficient mutants have less initial stability than the wild-type and fimbriae-deficient mutants, evidenced by their fluctuating position at equilibrium. TIRF observations at the microscopic level were complemented macroscopically using quartz crystal microbalance with dissipation (QCM-D) and sand-packed column experiments, which support the distinctive behavior observed at the microscopic scale. For each mutant strain, as fluorescence intensity increased in TIRF, the negative frequency shift in QCM-D (related to the attached mass of bacteria) also increased. Packed-column experiments indicated that curli- and flagella-deficient mutants exhibited a characteristically different attachment behavior and more retention as compared to the wild-type and fimbriae-deficient strains. This study utilized a new approach to understand bacterial attachment/detachment and provides new insights into the role of various appendages on initial attachment and stability
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Olsson, Adam L J
|e verfasserin
|4 aut
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|a Asadishad, Bahareh
|e verfasserin
|4 aut
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| 700 |
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|a van der Bruggen, Bart
|e verfasserin
|4 aut
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|a Tufenkji, Nathalie
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 33(2017), 16 vom: 25. Apr., Seite 4066-4075
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:33
|g year:2017
|g number:16
|g day:25
|g month:04
|g pages:4066-4075
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|u http://dx.doi.org/10.1021/acs.langmuir.7b00314
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