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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.6b04582
|2 doi
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|a pubmed24n0901.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Parveen, Nagma
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion
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|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 01.10.2018
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|a Date Revised 01.10.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Multivalent receptor-mediated interactions between virions and a lipid membrane can be weakened using competitive nonpathogenic ligand binding. In particular, the subsequent binding of such ligands can induce detachment of bound virions, a phenomenon of crucial relevance for the development of new antiviral drugs. Focusing on the simian virus 40 (SV40) and recombinant cholera toxin B subunit (rCTB), and using (monosialotetrahexosyl)ganglioside (GM1) as their common receptor in a supported lipid bilayer (SLB), we present the first detailed investigation of this phenomenon by employing the quartz crystal microbalance with dissipation (QCM-D) and total internal reflection fluorescence (TIRF) microscopy assisted 2D single particle tracking (SPT) techniques. Analysis of the QCM-D-measured release kinetics made it possible to determine the binding strength of a single SV40-GM1 pair. The release dynamics of SV40, monitored by SPT, revealed that a notable fraction of SV40 becomes mobile just before the release, allowing to estimate the distribution of SV40-bound GM1 receptors just prior to release
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Ligands
|2 NLM
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|a Lipid Bilayers
|2 NLM
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|a Phosphatidylcholines
|2 NLM
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|a G(M1) Ganglioside
|2 NLM
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| 650 |
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|a 37758-47-7
|2 NLM
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| 650 |
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|a Cholera Toxin
|2 NLM
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|a 9012-63-9
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|a 1-palmitoyl-2-oleoylphosphatidylcholine
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| 700 |
1 |
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|a Block, Stephan
|e verfasserin
|4 aut
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1 |
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|a Zhdanov, Vladimir P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rydell, Gustaf E
|e verfasserin
|4 aut
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| 700 |
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|a Höök, Fredrik
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 33(2017), 16 vom: 25. Apr., Seite 4049-4056
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:33
|g year:2017
|g number:16
|g day:25
|g month:04
|g pages:4049-4056
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|u http://dx.doi.org/10.1021/acs.langmuir.6b04582
|3 Volltext
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