A barley homolog of yeast ATG6 is involved in multiple abiotic stress responses and stress resistance regulation

A barley homolog of yeast ATG6 is involved in multiple abiotic stress responses and stress resistance regulation

Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 115(2017) vom: 06. Juni, Seite 97-106
1. Verfasser: Zeng, Xiaowei (VerfasserIn)
Weitere Verfasser: Zeng, Zhanghui, Liu, Cuicui, Yuan, Weiyi, Hou, Ning, Bian, Hongwu, Zhu, Muyuan, Han, Ning
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article ATG6 Abiotic stress Autophagy Barley PI3K VIGS Beclin-1
Beschreibung
Zusammenfassung:Copyright © 2017 Elsevier Masson SAS. All rights reserved.
Autophagy is a highly conserved degradation pathway among eukaryote cells, which can recycle damaged or unwanted cell materials upon encountering stress conditions. As a key component of the Class III PI3K kinase complex, ATG6/Beclin-1 is essential for autophagosome formation. In this study, we isolated a putative HvATG6 gene in barley genome. The protein encoded by HvATG6 shares high sequence identity to ATG6 orthologs in rice and wheat, and has a typical autophagy-specific domain containing segments of repeated β-sheet-α-helix. The expression of HvATG6 protein restored the appearance of autophagosomes in yeast atg6 mutant, indicating that HvATG6 complements the deficiency of yeast ATG6 protein in autophagy. Punctate florescence signals, considered as the PAS for autophagosome initiation, were observed in the cytoplasm of cells when HvATG6-GFP fusion construct was transformed into barley protoplast. Furthermore, the expression of HvATG6 was upregulated by various abiotic stresses including dark, H2O2 treatment, nitrogen deficiency, high salinity, drought, low temperature and toxic aluminum. Knockdown of HvATG6 in barley leaves through barley strip mosaic virus (BSMV)-induced gene silencing led to accelerated yellowing under dark and H2O2 treatments. Based on the above findings, we propose that barley ATG6 plays the similar role as other plant ATG6 orthologs, and might be involved in stress-induced autophagy process
Beschreibung:Date Completed 16.11.2017
Date Revised 13.12.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2017.03.013