StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato

© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 68(2017), 5 vom: 01. Feb., Seite 1265-1281
1. Verfasser: Zhou, Xiangjun (VerfasserIn)
Weitere Verfasser: Zha, Manrong, Huang, Jing, Li, Li, Imran, Muhammad, Zhang, Cankui
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Gene expression PHOSPHATE1 RNA-Seq StMYB44 phosphate deficiency potato protein–protein interaction Phosphate Transport Proteins mehr... Phosphates Plant Proteins Transcription Factors
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520 |a Phosphorus is an important macronutrient for plant growth, but often deficient in soil. To understand the molecular basis of the complex responses of potato (Solanum tuberosum L.) to phosphate (Pi) deficiency stress, the RNA-Seq approach was taken to identify genes responding to Pi starvation in potato roots. A total of 359 differentially expressed genes were identified, among which the Solanum tuberosum transcription factor gene MYB44 (StMYB44) was found to be down-regulated by Pi starvation. StMYB44 was ubiquitously expressed in potato tissues and organs, and StMYB44 protein was exclusively localized in the nucleus. Overexpression of StMYB44 in potato resulted in lower accumulation of Pi in shoots. Transcriptomic analysis indicated that the abundance of S. tuberosum PHOSPHATE1 (StPHO1), a Pi transport-related gene, was reduced in StMYB44 overexpression lines. In contrast, knock-out of StMYB44 by a CRISPR/Cas9 system failed to increase transcription of StPHO1. Moreover, StMYB44 was found to interact in the nucleus with AtWRKY6, a known Arabidopsis transcription factor directly regulating PHO1 expression, and StWRKY6, indicating that StMYB44 could be a member of the regulatory complex controlling transcription of StPHO1. Taken together, our study demonstrates that StMYB44 negatively regulates Pi transport in potato by suppressing StPHO1 expression 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Gene expression 
650 4 |a PHOSPHATE1 
650 4 |a RNA-Seq 
650 4 |a StMYB44 
650 4 |a phosphate deficiency 
650 4 |a potato 
650 4 |a protein–protein interaction 
650 7 |a Phosphate Transport Proteins  |2 NLM 
650 7 |a Phosphates  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
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700 1 |a Zha, Manrong  |e verfasserin  |4 aut 
700 1 |a Huang, Jing  |e verfasserin  |4 aut 
700 1 |a Li, Li  |e verfasserin  |4 aut 
700 1 |a Imran, Muhammad  |e verfasserin  |4 aut 
700 1 |a Zhang, Cankui  |e verfasserin  |4 aut 
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