Comparison of transcriptional expression patterns of carotenoid metabolism in 'Cabernet Sauvignon' grapes from two regions with distinct climate

Copyright © 2017 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 213(2017) vom: 15. Juni, Seite 75-86
1. Verfasser: Chen, Wei-Kai (VerfasserIn)
Weitere Verfasser: Yu, Ke-Ji, Liu, Bin, Lan, Yi-Bin, Sun, Run-Ze, Li, Qiang, He, Fei, Pan, Qiu-Hong, Duan, Chang-Qing, Wang, Jun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Cabernet Sauvignon Carotenoids Climatic conditions Norisoprenoids RNA-Seq Plant Proteins 36-88-4 Abscisic Acid 72S9A8J5GW mehr... Dioxygenases EC 1.13.11.- 9-cis-epoxy-carotenoid dioxygenase EC 1.13.11.51
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245 1 0 |a Comparison of transcriptional expression patterns of carotenoid metabolism in 'Cabernet Sauvignon' grapes from two regions with distinct climate 
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520 |a Copyright © 2017 Elsevier GmbH. All rights reserved. 
520 |a The downstream flux of carotenoid metabolism in grape berries includes the biosynthesis of norisoprenoids, a group of important aroma compounds, and the production of ABA, a well-known plant hormone. This study focused on the transcriptional profiling comparison of genes participating in the biosynthesis of carotenoids, norisoprenoids, and ABA in Vitis vinifera 'Cabernet Sauvignon' grapes at pea size, veraison, and ripening stages. The grapes were obtained from Changli (CL, eastern China) and Gaotai (GT, western China) regions and analyzed using RNA-sequencing technology. The transcripts required for the carotenoid biosynthesis pathway showed a coordinated expression pattern, mainly expressing at green stage for CL and at veraison for GT, respectively. However, the carotenoid content evolution was not coincident with the timing and pattern of related gene expressions, since more carotenoids were accumulated at veraison in CL relative to two weeks before veraison in GT. Interestingly, norisoprenoid content was higher in GT than in CL, particularly at veraison and ripening, while the key gene encoding carotenoid cleavage dioxygenases, VvCCD1, showed an inverse relationship within the two regions. Higher flux was expected through the carotenoid pathway into ABA production in GT, based on the higher expression level of 9-cis-epoxycarotenoid dioxygenase and drought growing conditions. Most components involved in ABA and ethylene signaling showed distinct expression profiles in the two regions. These results revealed that downstream flux of carotenoid metabolism in grape berries showed regional differences. This study lays a foundation for future research to explore the molecular basis of climatic influences on carotenoid, norisoprenoid, and ABA biosynthesis 
650 4 |a Journal Article 
650 4 |a Cabernet Sauvignon 
650 4 |a Carotenoids 
650 4 |a Climatic conditions 
650 4 |a Norisoprenoids 
650 4 |a RNA-Seq 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Carotenoids  |2 NLM 
650 7 |a 36-88-4  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
650 7 |a 72S9A8J5GW  |2 NLM 
650 7 |a Dioxygenases  |2 NLM 
650 7 |a EC 1.13.11.-  |2 NLM 
650 7 |a 9-cis-epoxy-carotenoid dioxygenase  |2 NLM 
650 7 |a EC 1.13.11.51  |2 NLM 
700 1 |a Yu, Ke-Ji  |e verfasserin  |4 aut 
700 1 |a Liu, Bin  |e verfasserin  |4 aut 
700 1 |a Lan, Yi-Bin  |e verfasserin  |4 aut 
700 1 |a Sun, Run-Ze  |e verfasserin  |4 aut 
700 1 |a Li, Qiang  |e verfasserin  |4 aut 
700 1 |a He, Fei  |e verfasserin  |4 aut 
700 1 |a Pan, Qiu-Hong  |e verfasserin  |4 aut 
700 1 |a Duan, Chang-Qing  |e verfasserin  |4 aut 
700 1 |a Wang, Jun  |e verfasserin  |4 aut 
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773 1 8 |g volume:213  |g year:2017  |g day:15  |g month:06  |g pages:75-86 
856 4 0 |u http://dx.doi.org/10.1016/j.jplph.2017.03.001  |3 Volltext 
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