Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus × Epinephelus lanceolatus

Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus × Epinephelus lanceolatus

© 2017 John Wiley & Sons Ltd.

Détails bibliographiques
Publié dans:Journal of fish diseases. - 1998. - 40(2017), 10 vom: 02. Okt., Seite 1351-1362
Auteur principal: Shen, G M (Auteur)
Autres auteurs: Shi, C Y, Fan, C, Jia, D, Wang, S Q, Xie, G S, Li, G Y, Mo, Z L, Huang, J
Format: Article en ligne
Langue:English
Publié: 2017
Accès à la collection:Journal of fish diseases
Sujets:Journal Article Vibrio harveyi antibiotic susceptibility grouper identification pathogenicity
Description
Résumé:© 2017 John Wiley & Sons Ltd.
The hybrid grouper, Epinephelus fuscoguttatus (♀) × Epinephelus lanceolatus (♂), is a newly bred cultivated marine fish species of high economic value. However, a skin ulcer disease with high mortality has occurred, and the responsible pathogen remains unknown. In this study, we summarized the epidemic status and external signs of this disease. We screened potential pathogens and finally isolated one bacterial strain ML01 from affected fish. We subjected healthy juvenile hybrid groupers to bacterial challenge tests with the isolate by immersion, immersion after dermal abrasion and intraperitoneal injection, respectively. Within 14 days post-infection, the isolate ML01 caused mass mortality of juveniles infected via immersion after dermal abrasion or intraperitoneal injection. Diseased juveniles displayed obvious signs of skin ulcers. The median lethal dose of ML01 by intraperitoneal injection was 1.10 × 105 colony-forming units. ML01 was identified as Vibrio harveyi by bacterial morphology, analytical profile index identification, 16S rDNA sequencing and multilocus sequence analysis. Antibiotic susceptibility tests showed that ML01 was sensitive to ceftriaxone, doxycycline and minocycline. The results of this study suggest that V. harveyi is the causal agent of skin ulcer disease in juvenile hybrid groupers, thus providing a basis for effective control and prevention of this disease
Description:Date Completed 14.05.2018
Date Revised 30.09.2020
published: Print-Electronic
GENBANK: KU232556, KU232559
Citation Status MEDLINE
ISSN:1365-2761
DOI:10.1111/jfd.12609