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231224s2017 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erw503
|2 doi
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|a pubmed24n0896.xml
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|a (DE-627)NLM268978468
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|a (NLM)28199682
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Hebelstrup, Kim H
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Waxy and non-waxy barley cultivars exhibit differences in the targeting and catalytic activity of GBSS1a
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|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 16.11.2017
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|a Date Revised 13.11.2018
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Amylose synthesis is strictly associated with activity of granule-bound starch synthase (GBSS) enzymes. Among several crops there are cultivars containing starch types with either little or no amylose known as near-waxy or waxy. This (near) amylose-free phenotype is associated with a single locus (waxy) which has been mapped to GBSS-type genes in different crops. Most waxy varieties are a result of either low or no expression of a GBSS gene. However, there are some waxy cultivars where the GBSS enzymes are expressed normally. For these types, single nucleotide polymorphisms have been hypothesized to represent amino-acid substitutions leading to loss of catalytic activity. We here confirm that the HvGBSSIa enzyme from one such waxy barley variety, CDC_Alamo, has a 90% reduction in catalytic activity. We also engineered plants with expression of transgenic C-terminal green fluorescent protein-tagged HvGBSSIa of both the non-waxy type and of the CDC_Alamo type to monitor their subcellular localization patterns in grain endosperm. HvGBSSIa from non-waxy cultivars was found to localize in discrete concentric spheres strictly within starch granules. In contrast, HvGBSSIa from waxy CDC_Alamo showed deficient starch targeting mostly into unknown subcellular bodies of 0.5-3 µm in size, indicating that the waxy phenotype of CDC_Alamo is associated with deficient targeting of HvGBSSIa into starch granules
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Amylose
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| 650 |
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4 |
|a GBSS
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| 650 |
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|a starch biosynthesis
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| 650 |
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4 |
|a starch functionality
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| 650 |
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4 |
|a subcellular targeting
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| 650 |
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4 |
|a waxy.
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Amylose
|2 NLM
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| 650 |
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|a 9005-82-7
|2 NLM
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| 650 |
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7 |
|a granule-bound starch synthase I
|2 NLM
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| 650 |
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7 |
|a EC 2.4.1.-
|2 NLM
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| 650 |
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|a Starch Synthase
|2 NLM
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| 650 |
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|a EC 2.4.1.21
|2 NLM
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| 700 |
1 |
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|a Nielsen, Morten Munch
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Carciofi, Massimiliano
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Andrzejczak, Olga
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shaik, Shahnoor Sultana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Blennow, Andreas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Palcic, Monica M
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 68(2017), 5 vom: 01. Feb., Seite 931-941
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:68
|g year:2017
|g number:5
|g day:01
|g month:02
|g pages:931-941
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erw503
|3 Volltext
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|d 68
|j 2017
|e 5
|b 01
|c 02
|h 931-941
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