TaqMan probes as blocking agents for enriched PCR amplification and DNA melting analysis of mutant genes
Asymmetric PCR and DNA melting analysis with TaqMan probes applied for mutation detection is effectively used in clinical diagnostics. The method is simple, cost-effective, and carried out in a closed-tube format, minimizing time, labor, and risk of sample cross-contamination. Although DNA melting a...
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Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 62(2017), 2 vom: 01. Feb., Seite 62-68
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| 1. Verfasser: |
Botezatu, Irina V
(VerfasserIn) |
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| Weitere Verfasser: |
Panchuk, Irina O,
Stroganova, Anna M,
Senderovich, Anastasia I,
Kondratova, Valentina N,
Shelepov, Valery P,
Lichtenstein, Anatoly V |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2017
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
BRAF
DNA melting analysis
NRAS
TaqMan probes
blocking agents
mutation detection
DNA Probes
Membrane Proteins
mehr...
DNA
9007-49-2
BRAF protein, human
EC 2.7.11.1
Proto-Oncogene Proteins B-raf
GTP Phosphohydrolases
EC 3.6.1.-
NRAS protein, human |
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